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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT5a antibody
See all STAT5a products (19) ...
Description
Rabbit polyclonal to STAT5a
Specificity
ab31377 detects endogenous levels of STAT5A protein around Tyrosine 694.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthesized non-phosphopeptide derived from human STAT5A around the phosphorylation site of Tyrosine 694.
Positive control
HeLa cell lysate, Breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS[without Calcium and Magnesium ions], 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10,000ELISA: 1/10,000
WB: 1/500 - 1/1000.Predicted molecular weight: 91 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accessionP42229
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Mammary gland factor antibody
- Mammary gland factor antibody
- MGF antibody
see all
Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
- SwissProt: Q62771 Rat
- Unigene: 437058 Human
see all
Anti-STAT5a antibody images:
Western blot - STAT5a antibody (ab31377)
Lane 1 : Anti-STAT5a antibody (ab31377) at 1/500 dilution
Lane 2 : Anti-STAT5a antibody (ab31377) at 1/500 dilution (preincubated with synthesized peptide)
Lane 1 : HeLa cell extract.
Lane 2 : HeLa cell extract.
Predicted band size : 91 kDa
Observed band size : 89 kDa (why is the actual band size different from the predicted?)
Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.
References for Anti-STAT5a antibody (ab31377)
ab31377 has not yet been referenced specifically in any publications.
Publishing research using ab31377? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"