Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518)
Key features and details
- Rabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody -
Description
Rabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730) -
Host species
Rabbit -
Specificity
ab194518 detects endogenous levels of STAT5a + STAT5b only when phosphorylated at serine 725 / serine 730. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human STAT5a (phospho S725) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
Database link: P42230
Synthetic peptide corresponding to Human STAT5b (phospho S730) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
Database link: P42232 -
Positive control
- EGF treated Jurkat cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab194518 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 91, 90 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 91, 90 kDa. |
Target
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Cellular localization
STAT5a: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. STAT5b: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. -
Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
- Unigene: 437058 Human
- Unigene: 595276 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab194518 has been referenced in 1 publication.
- You YH et al. MicroRNA-153 promotes brain-derived neurotrophic factor and hippocampal neuron proliferation to alleviate autism symptoms through inhibition of JAK-STAT pathway by LEPR. Biosci Rep 39:N/A (2019). PubMed: 30975733