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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Immunohistochemistry (Paraffin-embedded sections) - STAT5a (phospho S780) antibody (ab30649)
Western blot - STAT5a (phospho S780) antibody (ab30649)
All lanes : Anti-STAT5a (phospho S780) antibody (ab30649)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with synthesized phosphopeptide
Predicted band size : 91 kDa
Observed band size : 91 kDa
Immunocytochemistry/ Immunofluorescence-STAT5a (phospho S780) antibody(ab30649)
ICC/IF image of ab30649 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30649, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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