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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT5a (phospho S780) antibody
See all STAT5a products (19) ...
Description
Rabbit polyclonal to STAT5a (phospho S780)
Specificity
ab30649 detects endogenous levels of STAT5A only when phosphorylated at Serine 780.
Tested applications
ICC/IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of Serine 780.
Positive control
IHC: breast carcinoma WB: HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab30649 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accessionP42229
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Mammary gland factor antibody
- Mammary gland factor antibody
- MGF antibody
see all
Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
- SwissProt: Q62771 Rat
- Unigene: 437058 Human
see all
Anti-STAT5a (phospho S780) antibody images:
Western blot - STAT5a (phospho S780) antibody (ab30649)
All lanes : Anti-STAT5a (phospho S780) antibody (ab30649)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with synthesized phosphopeptide
Predicted band size : 91 kDa
Observed band size : 91 kDa
Immunocytochemistry/ Immunofluorescence-STAT5a (phospho S780) antibody(ab30649)
ICC/IF image of ab30649 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-STAT5a (phospho S780) antibody (ab30649)
ab30649 has not yet been referenced specifically in any publications.
Publishing research using ab30649? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"