Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)

Overview

  • Product name
    Anti-STAT5a (phospho Y694) antibody [6.F1]
    See all STAT5a primary antibodies
  • Description
    Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
  • Specificity
    ab106095 is specific for STAT5a protein phosphorylated at Y694.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Y694 of Mouse STAT5a protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab106095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 91 kDa.
IHC-P Use a concentration of 20 - 40 µg/ml.
ICC/IF 1/50 - 1/1000.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Anti-STAT5a (phospho Y694) antibody [6.F1] images

  • All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)

    Lane 1 : NK92 cell lysate, untreated
    Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)

    Secondary
    Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution

    Predicted band size : 91 kDa
  • ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
  • ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.

References for Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)

ab106095 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6 - using a pressure cooker
Permeabilization
Yes - Triton100-X
Specification
Mammary gland
Blocking step
MOM Mouse IgG blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Username

Flavia Ghiraldini

Verified customer

Submitted Sep 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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