STAT5a (phospho Y694) antibody (ab30648)

Product Name 

STAT5a (phospho Y694) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to STAT5a (phospho Y694)

Immunogen 

Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinoma tissue; HeLa cells treated with EGF.

Cellular localization 

Cytoplasmic, translocated into the nucleus in response to phosphorylation.

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

Relevance 

Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for STAT5a (phospho Y694) antibody images, references, products related to ab30648 and other tools.

STAT5a (phospho Y694) antibody images:

Customer reviews (feedback) regarding STAT5a (phospho Y694) antibody

Customer FAQs regarding STAT5a (phospho Y694) antibody

Protocols for STAT5a (phospho Y694) antibody

Price and availability of products related to STAT5a (phospho Y694) antibody

STAT5a (phospho Y694) antibody - related products: