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- STAT5a (phospho Y694) antibody (ab30648)
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Western blot - STAT5a (phospho Y694) antibody (ab30648)
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Product Name
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STAT5a (phospho Y694) antibody
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See all STAT5a antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to STAT5a (phospho Y694)
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Immunogen
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Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma tissue; HeLa cells treated with EGF.
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Cellular localization
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Cytoplasmic, translocated into the nucleus in response to phosphorylation.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
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Relevance
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Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.
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STAT5a (phospho Y694) antibody (ab30648) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this STAT5a (phospho Y694) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for STAT5a (phospho Y694) antibody images, references, products related to ab30648 and other tools.
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STAT5a (phospho Y694) antibody images:
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 - STAT5a (phospho Y694) antibody (ab30648)
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
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Western blot - STAT5a (phospho Y694) antibody (ab30648)
All lanes : STAT5a (phospho Y694) antibody (ab30648)
Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with EGF
Predicted band size : 91 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to STAT5a
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STAT5a (phospho Y694) antibody - more information
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Customer reviews (feedback) regarding STAT5a (phospho Y694) antibody |
Customer FAQs regarding STAT5a (phospho Y694) antibody |
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STAT5a (phospho Y694) antibody - related products:
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STAT5a (phospho Y694) antibody - other tools:
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Contact Abcam with a Technical Enquiry about STAT5a (phospho Y694) antibody |
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