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Anti-STAT5a (phospho Y694) antibody (ab30648)

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Overview

Product name

Anti-STAT5a (phospho Y694) antibody
See all STAT5a products (19) ...

Description

Rabbit polyclonal to STAT5a (phospho Y694)

Specificity

ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.

Positive control

Breast carcinoma tissue; HeLa cells treated with EGF.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT5a (phospho Y694) antibody (ab30648)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT5a (phospho Y694) antibody (ab30648) image (enlarge)

  • Western blot - STAT5a (phospho Y694) antibody (ab30648)Western blot - STAT5a (phospho Y694) antibody (ab30648) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab30648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.

Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

Target information above from: UniProt accessionP42229 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Mammary gland factor antibody
  • Mammary gland factor antibody
  • MGF antibody
  • MGF antibody
  • Signal transducer and activator of transcription 5A antibody
  • Signal transducer and activator of transcription 5A antibody
  • STA5A_HUMAN antibody
  • STAT 5 antibody
  • STAT 5A antibody
  • STAT5 antibody
  • STAT5 antibody
  • STAT5A antibody
see all

Database links

see all

Anti-STAT5a (phospho Y694) antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT5a (phospho Y694) antibody (ab30648)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT5a (phospho Y694) antibody (ab30648)

Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.

  Western blot - STAT5a (phospho Y694) antibody (ab30648)

Western blot - STAT5a (phospho Y694) antibody (ab30648)

All lanes : Anti-STAT5a (phospho Y694) antibody (ab30648)

Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with EGF


Predicted band size : 91 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-STAT5a (phospho Y694) antibody (ab30648)

This product has been referenced in:

  • Bertucci PYet al. Glucocorticoid-induced impairment of mammary gland involution is associated with STAT5 and STAT3 signaling modulation. Endocrinology 151:5730-40 (2010).Read more (PubMed: 20881248) »

See 1 publication for this product

Publishing research using ab30648? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"