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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT5b antibody
See all STAT5b products (12) ...
Description
Rabbit polyclonal to STAT5b
Specificity
ab52162 detects endogenous levels of total STAT5b protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human STAT5b around the phosphorylation site of serine 731(A-P-S-P-A).
Positive control
extracts from RAW264.7 cells, treated with EGF
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - STAT5b antibody (ab52162)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Involvement in disease
Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accessionP51692
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Transcription factor STAT5B antibody
- Signal transducer and activator of transcription 5B antibody
- STA5B_HUMAN antibody
see all
Database links
- Entrez Gene: 6777 Human
- Entrez Gene: 20851 Mouse
- Entrez Gene: 25126 Rat
- Omim: 604260 Human
- SwissProt: P51692 Human
- SwissProt: P42232 Mouse
- SwissProt: P52632 Rat
- Unigene: 434992 Human
see all
Anti-STAT5b antibody images:
Western blot - STAT5b antibody (ab52162)
All lanes : Anti-STAT5b antibody (ab52162) at 1/500 dilution
Lane 1 : extracts from RAW264.7 cells,
treated with EGF (200ng/ml 30min), without immunising peptide
Lane 2 : extracts from RAW264.7 cells,
treated with EGF (200ng/ml 30min), with immunising peptide
Predicted band size : 90 kDa
Observed band size : 90 kDa
References for Anti-STAT5b antibody (ab52162)
ab52162 has not yet been referenced specifically in any publications.
Publishing research using ab52162? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"