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Immunoprecipitation - STAT5b antibody (ab65743)
(enlarge)
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Product Name
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STAT5b antibody
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See all STAT5b antibodies (11)...
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Product type
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Primary antibodies
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Description
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Sheep polyclonal to STAT5b
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Immunogen
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Synthetic peptide: CMDSQWIPHAQS, corresponding to C terminal amino acids 777-787 of Human STAT5b conjugated to KLH
BLAST 'CMDSQWIPHAQS' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Chk, Cow, CynMk, Pig, Xl
(Due to sequence homology)
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Tested applications
(see key)
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IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2µg/mg of lysate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat liver lysate.
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Cellular localization
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Cytoplasmic and Nuclear: Translocated into the
nucleus in response to phosphorylation.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
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Relevance
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Stats (signal transducers and activators of transcription) are transcription factors that are activated by JAK family kinases. Stat1 and Stat2 are induced by IFN alpha and forn1 a heterodimer which is part of the ISGF-3 transcription factor complex. Stat3 beta appears to be activated by both EGF and IL6, but Stat3 alpha is activated by only by EGF. Stat4, predominantly expressed in testis and myeloid cells, is activated by ILl2. Stat 5 is activated by prolactin and IL3. Stat 6 participates in signaling pathways mediated by IL4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Sheep
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 150mM Sodium chloride, 10mM HEPES, pH 7.5 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this STAT5b antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for STAT5b antibody images, references, products related to ab65743 and other tools.
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STAT5b antibody images:
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 Immunoprecipitation - STAT5b antibody (ab65743)
Immunoprecipitation of ab65743 (2µg/mg lysate) with rat liver lysate using protein G beads (lane
1), and controls using beads alone (lane 2).
Western blot analysis by another commercially available STAT5b antibody.
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Search PubMed (MEDLINE) for references to STAT5b
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STAT5b antibody - more information
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Customer reviews (feedback) regarding STAT5b antibody |
Customer FAQs regarding STAT5b antibody |
Protocols for STAT5b antibody |
Price and availability of products related to STAT5b antibody |
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STAT5b antibody - related products:
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STAT5b antibody - other tools:
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Contact Abcam with a Technical Enquiry about STAT5b antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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