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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab76944? Please let us know so that we can cite the reference in this datasheet
ab76944 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - STAT5b antibody (ab76944)
Immunofluorescence analysis of HeLa cells using ab76944 at 10µg/ml.
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + immunogwen (GST-tagged 391aa recombinant full length human protein AAH20868) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 90 kDa
Observed band size : 69 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + HeLa nuclear lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 90 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-STAT5b antibody(ab76944)
ab76944 at 10 ug/ml staining STAT5b in human Hella cells by Immunocytochemistry/ Immunofluorescence.
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