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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - STAT5b antibody (ab76944)
Immunofluorescence analysis of HeLa cells using ab76944 at 10µg/ml.
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + immunogwen (GST-tagged 391aa recombinant full length human protein AAH20868) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 90 kDa
Observed band size : 69 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + HeLa nuclear lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 90 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-STAT5b antibody(ab76944)
ab76944 at 10 ug/ml staining STAT5b in human Hella cells by Immunocytochemistry/ Immunofluorescence.
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