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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT5b antibody
See all STAT5b products (12) ...
Description
Mouse monoclonal to STAT5b
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
GST-tagged recombinant full length Human protein (391aa, according to AAH20868)
Positive control
ICC/IF:HeLa cells; WB: HeLa cell nuclear cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 90 kDa.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Involvement in disease
Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accessionP51692
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Transcription factor STAT5B antibody
- Signal transducer and activator of transcription 5B antibody
- STA5B_HUMAN antibody
see all
Database links
- Entrez Gene: 6777 Human
- Omim: 604260 Human
- SwissProt: P51692 Human
- Unigene: 434992 Human
- Unigene: 595276 Human
Anti-STAT5b antibody images:
Immunocytochemistry/ Immunofluorescence - STAT5b antibody (ab76944)
Immunofluorescence analysis of HeLa cells using ab76944 at 10µg/ml.
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + immunogwen (GST-tagged 391aa recombinant full length human protein AAH20868) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 90 kDa
Observed band size : 69 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
Western blot - STAT5b antibody (ab76944)
Anti-STAT5b antibody (ab76944) at 1/500 dilution + HeLa nuclear lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 90 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-STAT5b antibody(ab76944)
ab76944 at 10 ug/ml staining STAT5b in human Hella cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-STAT5b antibody (ab76944)
ab76944 has not yet been referenced specifically in any publications.
Publishing research using ab76944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"