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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Anti-STAT5b (phospho S731) antibody (ab52211)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab52211? Please let us know so that we can cite the reference in this datasheet
ab52211 has been referenced in 1 publications.
- Chen AY et al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB; Human. PubMed: 21698228
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - STAT5b (phospho S731) antibody (ab52211)
All lanes : Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min)
Lane 2 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide
Predicted band size : 90 kDa
Immunohistochemistry (Paraffin-embedded sections) - STAT5b (phospho S731) antibody (ab52211)
Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide.
Immunocytochemistry/ Immunofluorescence-STAT5b (phospho S731) antibody(ab52211)
ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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