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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT5b (phospho S731) antibody
See all STAT5b products (12) ...
Description
Rabbit polyclonal to STAT5b (phospho S731)
Tested applications
ICC/IF, WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide based on human STAT5B around the phosphorylation site of serine 731 (A-P-SP-P-A).
Positive control
Human breast carcinoma tissue; extracts from RAW264.7 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C, Stable for 6 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Involvement in disease
Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accessionP51692
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Transcription factor STAT5B antibody
- Signal transducer and activator of transcription 5B antibody
- STA5B_HUMAN antibody
see all
Database links
- Entrez Gene: 6777 Human
- Entrez Gene: 20851 Mouse
- Entrez Gene: 25126 Rat
- Omim: 604260 Human
- SwissProt: P51692 Human
- SwissProt: P42232 Mouse
- SwissProt: P52632 Rat
- Unigene: 434992 Human
see all
Anti-STAT5b (phospho S731) antibody images:
Western blot - STAT5b (phospho S731) antibody (ab52211)
All lanes : Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min)
Lane 2 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide
Predicted band size : 90 kDa
Immunohistochemistry (Paraffin-embedded sections) - STAT5b (phospho S731) antibody (ab52211)
Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide.
Immunocytochemistry/ Immunofluorescence-STAT5b (phospho S731) antibody(ab52211)
ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-STAT5b (phospho S731) antibody (ab52211)
This product has been referenced in:
- Chen AYet al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB; Human.Read more (PubMed: 21698228) »
See 1 publication for this product
Publishing research using ab52211? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"