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ab126430 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT6 (Tyr641) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human cell lines. By determining STAT6 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the In-Cell STAT6 (Tyr641) ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT6 (Tyr641) or Anti-STAT6 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-Rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Mouse IgG Concentrate (Item I-2)||1 x 10µl|
|Anti-Rabbit IgG Concentrate (Item I-1)||1 x 10µl|
|Blocking Solution (5x) (Item F)||1 x 20ml|
|Fixing Solution (Item D)||1 x 30ml|
|Microplate (Item A)||1 unit|
|Mouse Anti-STAT6 Concentrate (Item H)||1 x 5µl|
|Quenching Buffer Concentrate (Item E)||1 x 2ml|
|Rabbit Anti-Phospho-STAT6 (Tyr641) Concentrate (Item G)||1 x 10µl|
|Stop Solution||1 x 14ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
|Wash Buffer Concentrate A (20x) (Item B)||1 x 30ml|
|Wash Buffer Concentrate B (20x) (Item C)||1 x 30ml|
Our Abpromise guarantee covers the use of ab126430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab126430 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"