You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-STAT6 antibody [YE361] (ab32520)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-STAT6 antibody [YE361]
See all STAT6 products (19) ...

Description

Rabbit monoclonal [YE361] to STAT6

Specificity

The antibody does not cross-react with other Stat family members.

Tested applications

WB, IHC-P, ICC/IF, IPmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues near C-terminus of human Stat-6.

Positive control

NIH 3T3 cell lysate, human skin carcinoma tissue and HeLa cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

YE361

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

  • Western blot - STAT6 antibody [YE361] (ab32520)Western blot - STAT6 antibody [YE361] (ab32520) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - STAT6 antibody [YE361] (ab32520)Immunohistochemistry (Paraffin-embedded sections) - STAT6 antibody [YE361] (ab32520) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - STAT6 antibody [YE361] (ab32520)Immunocytochemistry/ Immunofluorescence - STAT6 antibody [YE361] (ab32520) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000. Detects a ba...Read more →

    WB: 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250. Pe...Read more →

    IHC-P: 1/100 - 1/250. Perform heat-induced antigen retrieval with citrate buffer pH 6.0 before proceeding with the IHC staining protocol.

  • 1 Image

    ICC/IF

     ICC/IF: 1/100.

    ICC/IF: 1/100.

  • IP

     IP: 1/80.

    IP: 1/80.

Application notes

Is unsuitable for or Flow Cyt.

Target

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

Target information above from: UniProt accessionP42226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • D12S1644 antibody
  • IL 4 STAT antibody
  • IL-4 Stat antibody
  • IL4 STAT antibody
  • IL4 STAT antibody
  • Interleukin 4 Induced antibody
  • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
  • Signal Transducer And Activator Of Transcription 6 antibody
  • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
  • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
  • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
  • STAT 6 antibody
  • STAT interleukin4 induced antibody
  • STAT, interleukin4 induced antibody
  • Stat6 antibody
  • STAT6_HUMAN antibody
  • STAT6B antibody
  • STAT6C antibody
  • Transcription factor IL 4 STAT antibody
see all

Database links

see all

Anti-STAT6 antibody [YE361] images:

  Western blot - STAT6 antibody [YE361] (ab32520)

Western blot - STAT6 antibody [YE361] (ab32520)

Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution + NIH 3T3 cell lysate

Predicted band size : 94 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - STAT6 antibody [YE361] (ab32520)

Immunohistochemistry (Paraffin-embedded sections) - STAT6 antibody [YE361] (ab32520)

Immunohistochemical staining of paraffin-embedded human skin carcinoma using ab32520 at 1/100 dilution.

  Immunocytochemistry/ Immunofluorescence - STAT6 antibody [YE361] (ab32520)

Immunocytochemistry/ Immunofluorescence - STAT6 antibody [YE361] (ab32520)

Immunofluorescent staining of HeLa cells using ab32520 at 1/100 dilution.

References for Anti-STAT6 antibody [YE361] (ab32520)

ab32520 has not yet been referenced specifically in any publications.

Publishing research using ab32520? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"