Recombinant Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free (ab223128)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18278-265] to STAT6 (phospho Y641) - BSA and Azide free
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra), Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free
See all STAT6 primary antibodies -
Description
Rabbit monoclonal [EPR18278-265] to STAT6 (phospho Y641) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra), Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi whole cell lysate treated with 100 ng/ml interleukin 4 (IL-4) for 15 minutes. ICC/IF: Daudi cells treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes. Flow Cyt (intra): Daudi treated with 100 ng/ml IL-4 for 15 minutes. IP: Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.
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General notes
ab223128 is the carrier-free version of ab188080.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18278-265 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
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IP |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa). |
IP
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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Alternative names
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
Images
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Dot blot analysis of STAT6 (phospho Y641) labeled with ab188080 at 1/1000 dilution.
Lane 1: STAT6 (phospho Y641) phospho peptide.
Lane 2: STAT6 non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).
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Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells, treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes, or untreated, labeling STAT6 (phospho Y641) with ab188080 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
The expression increased and there was nuclear translocation of STAT6 after treatment with interleukin 4 (IL-4) (100 ng/ml) for 15minutes (PMID:22226123).
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Daudi (Human Burkitt's lymphoma cell line) treated/untreated with 100 ng/ml IL-4 for 15 minutes cells labeling STAT6 (phospho Y641) with ab188080 at 1/400 dilution (treated, red; untreated, green) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).
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STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg of Daudi (Human Burkitt's lymphoma cell line) whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes with ab188080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes, 10µg (Input). Lane 2: ab188080 IP in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188080 in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188080).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab223128 has not yet been referenced specifically in any publications.