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STEAP1 293T Transfected Lysate - (positive control) (ab94255)

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Overview

  • Product nameSTEAP1 293T Transfected Lysate - (positive control)
  • General notesab94255 is a 293T cell transfected lysate in which Human STEAP1 has been transiently over-expressed using a pCMV-STEAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Tissue specificity: Ubiquitously expressed. Highly expressed in prostate tumors. Similarity: Belongs to the STEAP family. Contains 1 ferric oxidoreductase domain.

    • Applications

    Our Abpromise guarantee covers the use of ab94255 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • STEAP1 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - STEAP1 293T Transfected Lysate - (positive control) (ab94255)
      SDS-PAGE - STEAP1 293T Transfected Lysate - (positive control) (ab94255)
      ab94255 at 15µg/lane on an SDS-PAGE gel
    • Western blot - STEAP1 293T Transfected Lysate - (positive control) (ab94255)
      Western blot - STEAP1 293T Transfected Lysate - (positive control) (ab94255)
      All lanes : Anti-STEAP1 antibody (ab57147) at 1/500 dilution

      Lane 1 : STEAP1 293T Transfected Lysate - (positive control) (ab94255)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for STEAP1 293T Transfected Lysate - (positive control) (ab94255)

    ab94255 has not yet been referenced specifically in any publications.

    Publishing research using ab94255 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"