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STEP / PTPN5 protein (Tagged-His Tag) (ab92052)

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Overview

  • Product nameSTEP / PTPN5 protein (Tagged-His Tag)See all STEP / PTPN5 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 443-565 of Human STEP / PTPN5with N terminal His tag; 123 amino acids, 23kDa (14kDa predicted from sequence). UniProt ID P54829
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 90 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesKSGTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAA QQEGPHCAP IIVHCSAGIGRTGCFIATSICCQQLRQEG VVDILKTTCQLRQDRGGMIQ TCEQYQFVHHVMSLYEKQ LSHQSPE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92052 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        FLJ14427Neural specific protein tyrosine phosphataseNeural-specific protein-tyrosine phosphatase
        Protein tyrosine phosphatase non receptor type 5Protein tyrosine phosphatase non receptor type 5 (striatum enriched)Protein tyrosine phosphatase striatum enrichedPTN5PTN5_HUMANPTP STEPPTPN 5Ptpn5PTPSTEPSTEPStriatum-enriched protein-tyrosine phosphataseTyrosine protein phosphatase non receptor type 5Tyrosine-protein phosphatase non-receptor type 5
      see all
  • FunctionMay regulates the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications    Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
  • Cellular localizationEndoplasmic reticulum membrane.

    • Target information above from: UniProt accession P54829 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    STEP / PTPN5 protein (Tagged-His Tag) images

    • SDS-PAGE - STEP / PTPN5 protein (Tagged-His Tag) (ab92052)
      SDS-PAGE - STEP / PTPN5 protein (Tagged-His Tag) (ab92052)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.

    References for STEP / PTPN5 protein (Tagged-His Tag) (ab92052)

    ab92052 has not yet been referenced specifically in any publications.

    Publishing research using ab92052 ? Please let us know so that we can cite the reference in this datasheet

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    Phone call requesting information relating to the reactivity of the STEP / PTPN5 proteins ab92052 and ab132095.

    Thank you for contacting us last week in regards to the STEP / PTPN5 protein.

    As discussed over the phone, I have checked into whether the enzymatic activity of either ab92052 or ab132095 has been established. Unfortunately, the activity of neith...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"