STEP / PTPN5 protein (Tagged-His Tag) (ab92052)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FLJ14427Neural specific protein tyrosine phosphataseNeural-specific protein-tyrosine phosphatase
- Protein tyrosine phosphatase non receptor type 5Protein tyrosine phosphatase non receptor type 5 (striatum enriched)Protein tyrosine phosphatase striatum enrichedPTN5PTN5_HUMANPTP STEPPTPN 5Ptpn5PTPSTEPSTEPStriatum-enriched protein-tyrosine phosphataseTyrosine protein phosphatase non receptor type 5Tyrosine-protein phosphatase non-receptor type 5
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
STEP / PTPN5 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.
References for STEP / PTPN5 protein (Tagged-His Tag) (ab92052)
ab92052 has not yet been referenced specifically in any publications.