Anti-STIL/SIL antibody (ab89314)
Key features and details
- Rabbit polyclonal to STIL/SIL
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-STIL/SIL antibody
See all STIL/SIL primary antibodies -
Description
Rabbit polyclonal to STIL/SIL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Gorilla, Opossum, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human STIL/SIL aa 1237-1287.
Database link: NP_003026.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 143 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Play a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. -
Tissue specificity
Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer. -
Involvement in disease
Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. -
Post-translational
modificationsPhosphorylated following the activation of the mitotic checkpoint. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 6491 Human
- Entrez Gene: 20460 Mouse
- Entrez Gene: 313506 Rat
- Omim: 181590 Human
- SwissProt: Q15468 Human
- SwissProt: Q60988 Mouse
- Unigene: 525198 Human
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Alternative names
- DKFZp686O09161 antibody
- MCPH7 antibody
- OTTHUMP00000009566 antibody
see all
Images
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All lanes : Anti-STIL/SIL antibody (ab89314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 143 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of STIL/SIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds. -
All lanes : Anti-STIL/SIL antibody (ab89314) at 0.1 µg/ml
Lane 1 : TCMK-1 cell lysate
Lane 2 : 4T1 cell lysate
Lane 3 : CT26.WT cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab89314 has been referenced in 26 publications.
- Huang F et al. Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing. J Biol Chem 298:102658 (2022). PubMed: 36356903
- Chi W et al. PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations. J Cell Biol 220:N/A (2021). PubMed: 33351100
- Gupta H et al. SAS-6 Association with ?-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. Curr Biol 30:2395-2403.e4 (2020). PubMed: 32442461
- So C et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 364:N/A (2019). PubMed: 31249032
- Gwon D et al. c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis. Mol Cells 42:840-849 (2019). PubMed: 31722512