Recombinant Anti-STIM2 antibody [EPR12879] - C-terminal (ab181258)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12879] to STIM2 - C-terminal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STIM2 antibody [EPR12879] - C-terminal
See all STIM2 primary antibodies -
Description
Rabbit monoclonal [EPR12879] to STIM2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A673 and BxPC3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12879 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
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Function
Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx. -
Tissue specificity
Expressed in all tissues and tumor cell lines examined. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsGlycosylated.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration. - Information by UniProt
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Database links
- Entrez Gene: 57620 Human
- Omim: 610841 Human
- SwissProt: Q9P246 Human
- Unigene: 726179 Human
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Alternative names
- FLJ39527 antibody
- KIAA1482 antibody
- STIM 2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181258 has been referenced in 1 publication.
- Pierro C et al. Oncogenic KRAS suppresses store-operated Ca2+ entry and ICRAC through ERK pathway-dependent remodelling of STIM expression in colorectal cancer cell lines. Cell Calcium 72:70-80 (2018). WB ; Human . PubMed: 29748135