Recombinant Anti-STIP1/STI1 antibody [EPR6605] (ab126724)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6605] to STIP1/STI1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-STIP1/STI1 antibody [EPR6605]
See all STIP1/STI1 primary antibodies -
Description
Rabbit monoclonal [EPR6605] to STIP1/STI1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, HEK-293, Jurkat, HeLa, BxPC3, HepG2, and SK OV 3 cell lysates, human, mouse and rat brain lysate. IHC-P: Human ovarian carcinoma, testis, thyroid cancer tissue. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6605 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
For unpurified use at1/20 |
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WB | (6) |
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
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Flow Cyt (Intra)
1/20. For unpurified use at1/20 |
WB
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
Sequence similarities
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
Domain
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
see all -
Alternative names
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
Images
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126724 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified)
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : SK OV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa -
Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purifiedab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution (Unpurified) + Mouse brain whole tissue lysate at 150 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 5 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab126724 has been referenced in 7 publications.
- Chen D et al. Comparative proteomics identify HSP90A, STIP1 and TAGLN-2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Exp Ther Med 23:374 (2022). PubMed: 35495589
- Siebert A et al. ALS-linked loss of Cyclin-F function affects HSP90. Life Sci Alliance 5:N/A (2022). PubMed: 36114006
- Beckley SJ et al. STIP1/HOP Regulates the Actin Cytoskeleton through Interactions with Actin and Changes in Actin-Binding Proteins Cofilin and Profilin. Int J Mol Sci 21:N/A (2020). PubMed: 32365744
- Cui J et al. Proteomic Identification of Pathways Responsible for the Estradiol Therapeutic Window in AD Animal Models. Front Cell Neurosci 13:437 (2019). PubMed: 31680862
- Guo X et al. STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway. Cancer Manag Res 11:10061-10072 (2019). PubMed: 31819639
- Göllner S et al. Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia. Nat Med 23:69-78 (2017). PubMed: 27941792
- He Y et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). PubMed: 27959342