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Anti-STIP1 antibody (ab65046) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 59 kDa (why is the actual band size different from the predicted?)
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
ab65046, at 4.0 µg/ml, staining STIP1 in human muscle tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained skeletal muscle cells.
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