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Products:Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Overview
Product name
Anti-STIP1 antibody
See all STIP1 products (9) ...
Description
Rabbit polyclonal to STIP1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide: QRCMMAQYNR HDSPEDVKRR AMADPEVQQI MSDPAMRLIL EQMQKDPQAL, corresponding to a region within C terminal amino acids 469-518 of Human STIP1
QRCMMAQYNR HDSPEDVKRR AMADPEVQQI MSDPAMRLIL EQMQKDPQAL
Positive control
Human muscle. Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 59 kDa (predicted molecular weight: 63 kDa).
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
Target
Function
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
Sequence similarities
Contains 2 STI1 domains.
Contains 9 TPR repeats.
Domain
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP31948
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hop antibody
- Hsc70/Hsp90 organizing protein antibody
- Hsc70/Hsp90-organizing protein antibody
see all
Database links
- Entrez Gene: 617109 Cow
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: Q3ZBZ8 Cow
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
see all
Anti-STIP1 antibody images:
Western blot - STIP1 antibody (ab65046)
Anti-STIP1 antibody (ab65046) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 59 kDa (why is the actual band size different from the predicted?)
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STIP1 antibody (ab65046)
ab65046, at 4.0 µg/ml, staining STIP1 in human muscle tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained skeletal muscle cells.
References for Anti-STIP1 antibody (ab65046)
This product has been referenced in:
- Heldens Let al. Co-chaperones are limiting in a depleted chaperone network. Cell Mol Life Sci 67:4035-48 (2010). WB; Human.Read more (PubMed: 20556630) »
See 1 publication for this product
Publishing research using ab65046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"