Anti-SPAK antibody (ab79045)
Key features and details
- Rabbit polyclonal to SPAK
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SPAK antibody
See all SPAK primary antibodies -
Description
Rabbit polyclonal to SPAK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human SPAK (internal sequence).
Database link: NP_037365 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity chromatography purified via peptide column -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. |
Target
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Function
May act as a mediator of stress-activated signals. -
Tissue specificity
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Domain
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins. -
Cellular localization
Cytoplasm. Nucleus. Nucleus when caspase-cleaved. - Information by UniProt
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Database links
- Entrez Gene: 54348 Rat
- SwissProt: O88506 Rat
- Unigene: 34414 Rat
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Alternative names
- DCHT antibody
- DKFZp686K05124 antibody
- OTTHUMP00000165175 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab79045 has been referenced in 2 publications.
- Sekii Y et al. Dietary salt with nitric oxide deficiency induces nocturnal polyuria in mice via hyperactivation of intrarenal angiotensin II-SPAK-NCC pathway. Commun Biol 5:175 (2022). PubMed: 35228649
- Yang HJ et al. Melatonin modulates nitric oxide-regulated WNK-SPAK/OSR1-NKCC1 signaling in dorsal raphe nucleus of rats. Korean J Physiol Pharmacol 25:449-457 (2021). PubMed: 34448462