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Overview

  • Product nameSTMN3 protein (Tagged-His Tag)See all STMN3 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 27-180 of Human STMN3 with N terminal His tag; 154 amino acids, 25.7kDa (18.1kDa predicted from sequence). UniProt ID = Q9NZ72.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 164 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTQPHPNTVYQYGDMEVKQLDKRASGQSFEVILKSPSDLSP ESPMLSSPPKKKDTSLE ELQKRLEAAEERRKTQEAQVL KQLAERREHEREVLHKALEENNNFSRQAEEKLNYKM EL SKEIREAHLAALRERLREKELHAAEVRRNKEQREEMSG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91814 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        9330161A03RikAI604839OTTHUMP00000031570
        OTTMUSP00000018010R75323RP23-83A14.1SCG10-like proteinScgn10 like proteinSclipStathmin like 3Stathmin-3STMN3STMN3_HUMAN
      see all
  • FunctionExhibits microtubule-destabilizing activity, which is antagonized by STAT3.
  • Tissue specificityNeuron specific.
  • Sequence similaritiesBelongs to the stathmin family.
  • Post-translational
    modifications    N-terminal palmitoylation promotes specific anchoring to the cytosolic leaflet of Golgi membranes and subsequent vesicular trafficking along dendrites and axons. Neuronal Stathmins are substrates for palmitoyltransferases ZDHHC3, ZDHHC7 and ZDHHC15.
  • Cellular localizationGolgi apparatus. Cell projection > growth cone. Cell projection > axon.

    • Target information above from: UniProt accession Q9NZ72 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    STMN3 protein (Tagged-His Tag) images

    • SDS-PAGE - STMN3 protein (Tagged-His Tag) (ab91814)
      SDS-PAGE - STMN3 protein (Tagged-His Tag) (ab91814)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for STMN3 protein (Tagged-His Tag) (ab91814)

    ab91814 has not yet been referenced specifically in any publications.

    Publishing research using ab91814 ? Please let us know so that we can cite the reference in this datasheet

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