Recombinant Anti-Stomatin antibody [EPR10421] (ab166623)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10421] to Stomatin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Stomatin antibody [EPR10421]
See all Stomatin primary antibodies -
Description
Rabbit monoclonal [EPR10421] to Stomatin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Stomatin.
Database link: P27105 -
Positive control
- Human fetal heart, HeLa, HepG2, Neuro-2a and Raw264.7 lysates, HeLa cells, permeabilized Neuro-2a cells, immunoprecipitation pellet from fetal heart lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10421 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Function
Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 2040 Human
- Entrez Gene: 13830 Mouse
- Entrez Gene: 296655 Rat
- Omim: 133090 Human
- SwissProt: P27105 Human
- SwissProt: P54116 Mouse
- Unigene: 253903 Human
- Unigene: 295284 Mouse
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Alternative names
- BND7 antibody
- EPB7 antibody
- EPB72 antibody
see all
Images
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All lanes : Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Neuro-2a lysate
Lane 5 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa -
Immunofluorescence analysis of HeLa cells labeling Stomatin with ab166623 at 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
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Immunoprecipitation of Stomatin in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of Stomatin.
Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution + immunoprecipitation pellet from Human fetal heart lysate at 10 µg
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab166623 has been referenced in 2 publications.
- Cao Y et al. Proteomic analysis identifies Stomatin as a biological marker for psychological stress. Neurobiol Stress 22:100513 (2023). PubMed: 36636173
- Wu K & Wang L Stomatin-knockdown effectively attenuates sepsis-induced oxidative stress and inflammation of alveolar epithelial cells by regulating CD36. Exp Ther Med 23:69 (2022). PubMed: 34934440