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Product name
Anti-SUZ12 antibody - ChIP Grade
See all SUZ12 products (5) ...
Description
Rabbit polyclonal to SUZ12 - ChIP Grade
Specificity
Antibody has been shown to recognise endogenous Suz12 in Colon cancer and Breast cancer cell line (See image below)
Tested applications
IP, ChIP/Chip, WB, ChIP, RIPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human SUZ12.
(Peptide available as ab123 89.)
Positive control
This antibody gave a positive signal when tested with: Lysates from HEK293 cells overexpressing Suz12; SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate; MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate; Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ChIP/Chip: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ChIP: Use at an assay dependent dilution.
RIP: Use at an assay dependent dilution. PubMed: 19571010
Target
Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Tissue specificity
Overexpressed in breast and colon cancer.
Involvement in disease
Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
Sequence similarities
Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
Contains 1 C2H2-type zinc finger.
Developmental stage
Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15022
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ChET 9 protein antibody
- CHET9 antibody
- Chromatin precipitated E2F target 9 protein antibody
see all
Database links
- Entrez Gene: 23512 Human
- Entrez Gene: 52615 Mouse
- GenBank: D63881 Human
- Omim: 606245 Human
- SwissProt: Q6ZN18 Human
- SwissProt: Q15022 Human
- SwissProt: Q80U70 Mouse
- Unigene: 126497 Human
see all
Anti-SUZ12 antibody - ChIP Grade images:
Western blot - SUZ12 antibody (ab12073)
All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 0.4 µg/ml
Lane 1 : Untransfected 293T cells
Lane 2 : 293T cells transfected with 5ug HA-SUZ12
developed using the ECL technique
Predicted band size : 75 kDa
Observed band size : 75 kDa
In addition to the Suz12 band shown, three other bands of higher MW were present in both lanes. These bands may be due to cross-reactivity of the antibody.
Western blot - SUZ12 antibody - ChIP Grade (ab12073)
All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 1 µg/ml
Lane 1 : untransfected 293T cell lysate
Lane 2 : 293T cells transfected with 5ug HA-Suz12
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 75 kDa
Observed band size : 76 kDa (why is the actual band size different from the predicted?)
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Western blot - SUZ12 antibody - ChIP Grade (ab12073)
All lanes : Anti-SUZ12 antibody - ChIP Grade (ab12073) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 30 kDa,53 kDa,60 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
ChIP - SUZ12 antibody - ChIP Grade (ab12073)
Chromatin was prepared from the Human Foreskin Fibroblasts cells. The cross-linking (X-ChiP) technique was used, crosslinking was done for 9 minutes in serum free 1% formaldehyde reagent. The primary antibody was diluted 1/500 in phosphate buffered ChiP dilution buffer and incubated with the sample for 16 hours at 4°C. HOX C13 region was used on the positive control, whilst normal rabbit IgG and GAPDH was used in the negative control. The immunoprecipitated DNA was quantified by real time PCR.
This image is courtesy of an anonymous Abreview
ChIP - SUZ12 antibody - ChIP Grade (ab12073)
Chromatin was prepared from nuclear lysate of the human MDA-MB-231 breast epithelial adenocarcinoma cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. The primary antibody was used in concentration of 0.2 µg/µg chromatin and incubated with the sample for 16 hours at 4°C in SDS, DOC, TritonX-100, EDTA, HEPES, NaCl. The immunoprecipitated DNA was quantified by real time PCR. Ct values were converted to DNA copy numbers using a standard curve in the Q-PCR step. The number of binding events detected for each test reaction was then calculated by taking into account the DNA copy number, cell equivalents of chromatin used in the ChIP and PCR, and primer pair amplification efficiency.
This image is a courtesy of Genpathway Inc
References for Anti-SUZ12 antibody - ChIP Grade (ab12073)
This product has been referenced in:
- Kim YW & Kim A Characterization of histone H3K27 modifications in the ß-globin locus. Biochem Biophys Res Commun 405:210-5 (2011). ChIP; Human.Read more (PubMed: 21219849) »
- Zeng Y et al. p53 binds to and is required for the repression of Arf tumor suppressor by HDAC and polycomb. Cancer Res 71:2781-92 (2011).Read more (PubMed: 21447739) »
See all 20 publications for this product
Publishing research using ab12073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"