Anti-SUZ12 antibody [SUZ220A] (ab126577)

Overview

• Product nameAnti-SUZ12 antibody [SUZ220A]See all SUZ12 primary antibodies ...
• Description
  Mouse monoclonal [SUZ220A] to SUZ12
• Tested applicationsIHC-Fr, WB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant full length protein (Human)

• Positive controlIHC: Human tonsil, testicle and thymus tissues; Mouse testicle tissue; V5-tagged Human SUZ12 expressed in Hek293T
  ICC/IF: Human tonsil
  WB: Hek-SUZ12-V5, HeLa, Jeko1, MT-2 and U266 cell lysates

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferConstituent: 99% Tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberSUZ220A
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation
  • Lysine methylation

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • HOX

Applications

Target

• FunctionPolycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
• Tissue specificityOverexpressed in breast and colon cancer.
• Involvement in diseaseNote=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
• Sequence similaritiesBelongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
  Contains 1 C2H2-type zinc finger.
• Developmental stageExpressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q15022 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23512 Human
  • Entrez Gene: 52615 Mouse
  • Omim: 606245 Human
  • SwissProt: Q15022 Human
  • SwissProt: Q80U70 Mouse
  • Unigene: 462732 Human
  • Unigene: 283410 Mouse
  • Unigene: 473315 Mouse

• Alternative names
  ChET 9 protein antibodyCHET9 antibodyChromatin precipitated E2F target 9 protein antibody

  JJAZ1 antibodyJoined to JAZF1 protein antibodyKIAA0160 antibodyPolycomb protein SUZ12 antibodySuppressor of zeste 12 homolog antibodySuppressor of zeste 12 protein homolog antibodySUZ12 antibodySUZ12_HUMAN antibody

  see all

Anti-SUZ12 antibody [SUZ220A] images

• 

  Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577) at 1/100 dilution
  
  Lane 1 : Hek-SUZ12-V5 lysate at 30 µg
  Lane 2 : Hek-mTIMP2 lysate at 30 µg
  Lane 3 : Hela cell line lysate at 200 µg
  Lane 4 : Jeko1 cell line lysate at 200 µg
  Lane 5 : Human tonsil lysate at 200 µg
  Lane 6 : Human brain lysate at 200 µg
  Lane 7 : MT-2 cell line lysate at 200 µg
  Lane 8 : 3T3 Mouse cell line lysate at 200 µg
  Lane 9 : U266 cell line lysate at 200 µg
  
  
  Predicted band size : 83 kDa
  Observed band size : 83 kDa
  
• 

  Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577)
  
  Lane 1 : Empty vector
  Lane 2 : shSUZ12.783
  Lane 3 : shSUZ12.2076
  Lane 4 : Empty vector
  Lane 5 : shSUZ12.783
  Lane 6 : shSUZ12.2076
  
  
  Predicted band size : 83 kDa
  Observed band size : 83 kDa
  
• 

  Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  Purified version of ab126577, at 1/500, staining SUZ12 in frozen Human tonsil tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  ab126577, at a 1/30 dilution, staining SUZ12 in paraffin embedded Mouse testicle tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human testicle tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  Purified ab126577 at 1/500 staining SUZ12 in paraffin embedded Human thymus tissue by immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  Purified version of ab126577, at a 1/100 dilution, staining SUZ12 (green) in Human tonsil by Immunofluorescence. IgD antibody (red) was used at 1/50. After antigen retrieval (Tris-EDTA buffer), the slides were incubated for one hour at room temperature in a humid chamber with primary antibodies. Slides were then washed in PBS 0.5%-Tween20 3 times for 5 minutes each. The slides were incubated for 1 hour with fluorochrome-conjugated antibodies against the different Ig isotypes, diluted in PBS (dilution 1/200) in a humid chamber in the dark. Subsequently, slides were washed in PBS 0.5% Tween20 3 times for 5 minutes each. Following washing, antifading was added.
• 

  Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  To confirm that ab126577 recognized Human SUZ12 protein, Immunohistochemistry on frozen cytospins preparations of V5-tagged Human SUZ12 expressed in Hek293T was performed. Labeling with the ab126577 confirmed the efficiency of transfection. Cytospin preparation of V5-tagged Human BTLA protein was used as a negative control.