Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
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ab16879 has been referenced in 4 publications.
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ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
This image is courtesy of an anonymous Abreview
All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
HRP conjugated sheep-anti mouse IgG
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 82 kDa
Observed band size : ~80 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 seconds
This image is courtesy of an Abreview submitted by Mr Adam Hoffhines
CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.
Image courtesy of Nir Drayman by Abreview.
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