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Immunocytochemistry/ Immunofluorescence - SV40 T-antigen antibody [PAb416] (ab16879)
![Immunocytochemistry/ Immunofluorescence - SV40 T-antigen antibody [PAb416] (ab16879)](/ps/datasheet/images/ab16879_1.jpg)
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Western blot - SV40 T-antigen antibody [PAb416] (ab16879)
![Western blot - SV40 T-antigen antibody [PAb416] (ab16879)](/ps/datasheet/images/ab16879_2.jpg)
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Product Name
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SV40 T-antigen antibody [PAb416]
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See all SV40 T-antigen antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PAb416] to SV40 T-antigen
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Immunogen
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SV40 transformed mouse cells
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Species Reactivity
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SV40 infected cells.
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Epitope
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Within amino acids 83-128 of the large T-antigen.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC (IF): Use at a concentration of 1 - 5 µg/ml.
IP: Use at a concentration of 1 - 5 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SV80 or SV-T2 cells.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Viral proteins >> SV40 antigen
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
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Relevance
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SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PAb416
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Myeloma
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NS1
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Isotype
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IgG2a
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SV40 T-antigen antibody [PAb416] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SV40 T-antigen antibody [PAb416] images, references, products related to ab16879 and other tools.
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SV40 T-antigen antibody [PAb416] images:
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 Immunocytochemistry/ Immunofluorescence - SV40 T-antigen antibody [PAb416] (ab16879)
ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF. This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - SV40 T-antigen antibody [PAb416] (ab16879)
All lanes : SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
HRP conjugated sheep-anti mouse IgG
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 82 kDa
Observed band size : ~80 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 seconds
This image is courtesy of an Abreview submitted by Mr Adam Hoffhines
See Abreview
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Search PubMed (MEDLINE) for references to SV40 T-antigen
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SV40 T-antigen antibody [PAb416] - more information
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Customer reviews (feedback) regarding SV40 T-antigen antibody [PAb416] |
Customer FAQs regarding SV40 T-antigen antibody [PAb416] |
Protocols for SV40 T-antigen antibody [PAb416] |
Price and availability of products related to SV40 T-antigen antibody [PAb416] |
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SV40 T-antigen antibody [PAb416] - related products:
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SV40 T-antigen antibody [PAb416] - other tools:
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Contact Abcam with a Technical Enquiry about SV40 T-antigen antibody [PAb416] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - SV40 T-antigen antibody [PAb416] (ab16879)](/index.html?datasheet=16879#ab16879_1.jpg)
![Western blot - SV40 T-antigen antibody [PAb416] (ab16879)](/index.html?datasheet=16879#ab16879_2.jpg)
