Anti-SV40 T-antigen antibody [PAb416] (ab16879)

  Immunocytochemistry/ Immunofluorescence - SV40 T-antigen antibody [PAb416] (ab16879)

 ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.

This image is courtesy of an anonymous Abreview

See Abreview

  Western blot - SV40 T-antigen antibody [PAb416] (ab16879)

All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml

Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

Lysates/proteins at 7 µg per lane.

Secondary
HRP conjugated sheep-anti mouse IgG
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 82 kDa
Observed band size : ~80 kDa (why is the actual band size different from the predicted?)


Exposure time : 2 seconds

This image is courtesy of an Abreview submitted by Mr Adam Hoffhines

See Abreview

  Flow Cytometry - SV40 T-antigen antibody [PAb416] (ab16879)

CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.

Image courtesy of Nir Drayman by Abreview.

See Abreview