Anti-SV40 T-antigen antibody [PAb416] (ab16879)

Overview

  • Product nameAnti-SV40 T-antigen antibody [PAb416]
    See all SV40 T-antigen primary antibodies
  • Description
    Mouse monoclonal [PAb416] to SV40 T-antigen
  • Tested applicationsICC, Flow Cyt, ICC/IF, WB, IPmore details
  • Species reactivity
    SV40 infected cells.
  • Immunogen

    SV40 transformed mouse cells

  • EpitopeWithin amino acids 83-128 of the large T-antigen.
  • Positive control
    • SV80 or SV-T2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 - 5 µg/ml.
Flow Cyt 1/100.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IP Use a concentration of 1 - 5 µg/ml.

Target

  • Cellular localizationNuclear
  • Alternative names
    • Large T antigen antibody
    • LT AG antibody
    • LT antibody
    • LTAG antibody
    • Small T antigen antibody
    see all

Anti-SV40 T-antigen antibody [PAb416] images

  •  ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.

    See Abreview

  • All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml

    Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
    Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

    Lysates/proteins at 7 µg per lane.

    Secondary
    HRP conjugated sheep-anti mouse IgG
    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : ~80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This image is courtesy of an Abreview submitted by Mr Adam Hoffhines

    See Abreview

  • CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.

    See Abreview

References for Anti-SV40 T-antigen antibody [PAb416] (ab16879)

This product has been referenced in:
  • Sharma BN  et al. Antiviral effects of artesunate on polyomavirus BK replication in primary human kidney cells. Antimicrob Agents Chemother 58:279-89 (2014). Read more (PubMed: 24145549) »
  • Henriksen S  et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus. J Virol 88:7556-68 (2014). Read more (PubMed: 24760884) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample African Green Monkey Cell (Simian virus 40 (SV40))
Specification Simian virus 40 (SV40)
Fixation Methanol
Permeabilization Yes - Methanol
Username

Mr. Nir Drayman

Verified customer

Submitted Aug 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount 10 µg
Specification Human Renal Proximal Tubular cells
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Robert Jenkins

Verified customer

Submitted Jun 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast tumor cell)
Loading amount 10 µg
Specification fibroblast tumor cell
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Mr. Manish Garg

Verified customer

Submitted Oct 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (SPLEEN)
Loading amount 30 µg
Specification SPLEEN
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts (MEFs))
Loading amount 7 µg
Specification Mouse Embryonic Fibroblasts (MEFs)
Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Adam Hoffhines

Verified customer

Submitted Mar 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (transfected keratinocyte)
Specification transfected keratinocyte
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 08 2006

Thank you for your enquiry. This clone was originally characterized in the following publication, Harlow, E., et al. (1981. J. Virol. 39:861, free on-line), the authors originally used this antibody (referred to as L16 in the original publication) to i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"