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Product Name
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SV40 T-antigen antibody [PAb419]
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See all SV40 T-antigen antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PAb419] to SV40 T-antigen
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Immunogen
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Full length protein (SV40).
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Species Reactivity
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SV40 infected cells.
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Epitope
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The N-terminal domain of the large and small T-antigen.
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Tested applications
(see key)
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ICC, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC (IF): Use at a concentration of 1 - 5 µg/ml.
IP: Use at a concentration of 1 - 2 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SV80 or SV-T2 cells.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Viral proteins >> SV40 antigen
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
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Relevance
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SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PAb419
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Myeloma
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NS1
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SV40 T-antigen antibody [PAb419] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for SV40 T-antigen antibody [PAb419] (ab16848)
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This product has been used in:
McLaughlin-Drubin ME et al. The Human Papillomavirus Type 16 E7 Oncoprotein Associates with E2F6. J Virol : (2008). PubMed: 18579589
If you publish research using ab16848 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SV40 T-antigen
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