Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Overview
Product name
SW1353 (Human bone chondrosarcoma, fibroblast-like cell line) Whole Cell Lysate
General notes
Cell line: SW1353 (Human humerus chondrosarcoma)br>Growth media: DMEM & 10% FBS (Fetal bovine serum)
Tested applications
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Lysate notes
SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Background
The SW1353 chondrosarcoma cell line is considered to be a model for studies of the transcription factor Sox9, an important determinant of chondrocyte differentiation, including the regulation of type II collagen (Col2) and aggrecan gene expression. Cells are used to study mechanisms of joint inflammation and cartilage destruction.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
SW1353 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane isrecommended for mini gel.
References for SW1353 (Human bone chondrosarcoma, fibroblast-like cell line) Whole Cell Lysate (ab7915)
ab7915 has not yet been referenced specifically in any publications.
Publishing research using ab7915? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"