SYBL1 antibody [158.2] (ab36195)

Product Name 

SYBL1 antibody [158.2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [158.2] to SYBL1

Immunogen 

Recombinant full SYBL1 protein (Human)

Reacts with 

Hu, Rat

Predicted to react 
(species key)

Ms, Dog
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution (PMID 19138172).
IHC-Fr: 1/10 - 1/400.
IHC-P: 1/10 - 1/400.
WB: 1/500 - 1/2000. Predicted molecular weight: 24 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

extract from rat brain

Cellular localization 

Membrane; single-pass type IV membrane protein

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs

Relevance 

SYBL1 (Synaptobrevin-like 1) involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.

Found ubiquitously and is an unusual v-SNAREs molecule implicated in cellular exocytosis. The SYBL1 gene is found on the pseudoautosomal region (PAR) of X chromosome. It is a highly conserved gene and has been shown to be silenced on inactive X and Y chromosomes.
In ICF syndrome, a human disease affecting DNA methylation, SYBL1 escapes from silencing and this correlates with the observed altered patterns of histone methylation and acetylation found. An association between some SYBL1 alleles and bipolar affective disorder (BPAD) has been observed.

SUBUNIT: Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

158.2

Isotype 

IgG1

Light chain type 

kappa

Purity 

Ascites

Storage buffer 

Preservative: None
Constituents: Ascites

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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References for SYBL1 antibody [158.2] (ab36195)

  This product has been used in: (two most recent references)

Ha SD et al. Cathepsin B-mediated autophagy flux facilitates the anthrax toxin receptor 2-mediated delivery of anthrax lethal factor into the cytoplasm. J Biol Chem 285:2120-9 (2010). PubMed: 19858192

Flowerdew SE & Burgoyne RD A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels. Biochem J 418:529-40 (2009). ICC/IF; Human. PubMed: 19138172

See all 5 publication references for this product.


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