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Anti-SYBL1 antibody [158.2] (ab36195)

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Overview

Product name

Anti-SYBL1 antibody [158.2]
See all SYBL1 products (6) ...

Description

Mouse monoclonal [158.2] to SYBL1

Tested applications

Flow Cyt, ICC/IF, WB, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse, Dog

Immunogen

Recombinant full SYBL1 protein (Human)

Positive control

extract from rat brain

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

158.2

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs

  • Western blot - SYBL1 antibody [158.2] (ab36195)Western blot - SYBL1 antibody [158.2] (ab36195) image (enlarge)

  • Flow Cytometry-SYBL1 antibody [158.2](ab36195)Flow Cytometry-SYBL1 antibody [158.2](ab36195) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.

Tissue specificity

Detected in all tissues tested.

Sequence similarities

Belongs to the synaptobrevin family.
Contains 1 longin domain.
Contains 1 v-SNARE coiled-coil homology domain.

Cellular localization

Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.

Target information above from: UniProt accessionP51809 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ53045 antibody
  • FLJ53762 antibody
  • FLJ54296 antibody
  • HGNC:11486 antibody
  • OTTHUMP00000024258 antibody
  • OTTHUMP00000024259 antibody
  • OTTHUMP00000225953 antibody
  • SYBL 1 antibody
  • SYBL1 antibody
  • Synaptobrevin like 1 antibody
  • Synaptobrevin-like protein 1 antibody
  • Tetanus insensitive VAMP antibody
  • Tetanus neurotoxin insensitive VAMP antibody
  • Tetanus-insensitive VAMP antibody
  • TI VAMP antibody
  • Ti-VAMP antibody
  • TIVAMP antibody
  • VAMP-7 antibody
  • VAMP7 antibody
  • VAMP7_HUMAN antibody
  • Vesicle-associated membrane protein 7 antibody
see all

Database links

see all

Anti-SYBL1 antibody [158.2] images:

  Western blot - SYBL1 antibody [158.2] (ab36195)

Western blot - SYBL1 antibody [158.2] (ab36195)

Anti-SYBL1 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain

Predicted band size : 24 kDa

  Flow Cytometry-SYBL1 antibody [158.2](ab36195)

Flow Cytometry-SYBL1 antibody [158.2](ab36195)

Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

References for Anti-SYBL1 antibody [158.2] (ab36195)

This product has been referenced in:

  • Tamura Ket al. Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes. J Biol Chem 286:7507-21 (2011).Read more (PubMed: 21187289) »
  • Ha SDet al. Cathepsin B-mediated autophagy flux facilitates the anthrax toxin receptor 2-mediated delivery of anthrax lethal factor into the cytoplasm. J Biol Chem 285:2120-9 (2010).Read more (PubMed: 19858192) »

See all 7 publications for this product

Publishing research using ab36195? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"