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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Overview
Product name
Anti-SYBL1 antibody
See all SYBL1 products (6) ...
Description
Rabbit polyclonal to SYBL1
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide as a part of human, rat, mouse, chimpanzee, xenopus, zebrafish (and other species) SYBL1 conjugated to an immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Immunocytochemistry/ Immunofluorescence-SYBL1 antibody(ab68834)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab68834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: Use a concentration of 1 - 10 µg/ml.Predicted molecular weight: 24 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
IHC-P: Use a concentration of 1 - 10 µg/ml.
IHC-Fr: Use a concentration of 1 - 10 µg/ml.
Target
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
Tissue specificity
Detected in all tissues tested.
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 longin domain.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localization
Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
Target information above from: UniProt accessionP51809
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ53045 antibody
- FLJ53762 antibody
- FLJ54296 antibody
see all
Database links
- Entrez Gene: 6845 Human
- Entrez Gene: 20955 Mouse
- Entrez Gene: 85491 Rat
- Omim: 300053 Human
- SwissProt: P51809 Human
- SwissProt: P70280 Mouse
- SwissProt: Q9CW09 Mouse
- SwissProt: Q9JHW5 Rat
see all
Anti-SYBL1 antibody images:
Immunocytochemistry/ Immunofluorescence-SYBL1 antibody(ab68834)
ICC/IF image of ab68834 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SYBL1 antibody (ab68834)
ab68834 has not yet been referenced specifically in any publications.
Publishing research using ab68834? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"