Anti-SYT8 antibody - N-terminal (ab185343)
Key features and details
- Rabbit polyclonal to SYT8 - N-terminal
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SYT8 antibody - N-terminal -
Description
Rabbit polyclonal to SYT8 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SYT8 aa 1-100 (N terminal).
Database link: Q8NBV8 -
Positive control
- IHC-P: Human soft tissue; ICC: Caco-2 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
|
IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Not known. As it is truncated compared to orthologs, its function is not obvious. -
Sequence similarities
Belongs to the synaptotagmin family. -
Cellular localization
Cell membrane. Cytoplasmic vesicle > secretory vesicle > acrosome. - Information by UniProt
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Database links
- Entrez Gene: 90019 Human
- Omim: 607719 Human
- SwissProt: Q8NBV8 Human
- Unigene: 161031 Human
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Alternative names
- Synaptotagmin VIII antibody
- Synaptotagmin-8 antibody
- Syt8 antibody
see all
Images
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Immunohistochemical analysis of human soft tissue labeling SYT8 in the cytoplasm of peripheral nerve with ab185343 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry analysis of Caco-2 (human colorectal adenocarcinoma cell line) whole cells labeling SYT8 in the nucleoplasm and cell junctions with ab185343 at 2 µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab185343 has not yet been referenced specifically in any publications.