Anti-SYTL2/SLP2 antibody (ab231133)
Key features and details
- Rabbit polyclonal to SYTL2/SLP2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SYTL2/SLP2 antibody -
Description
Rabbit polyclonal to SYTL2/SLP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human SYTL2/SLP2 aa 329-880. Expressed in E.coli. N-terminal tags.
Database link: Q9HCH5 -
Positive control
- WB: Recombinant human SYTL2/SLP2; mouse cerebrum tissue lysate; pig cerebrum tissue lysate. IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
Target
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Function
Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon-containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca(2+) while binding to PIP2 is Ca(2+) insensitive. -
Tissue specificity
Isoform 1 is expressed in hematopoietic lineages with a strong expression in CD4 and CD8 T-lymphocytes. It is also widely expressed in nonhematopoietic tissues. Isoform 5 is expressed only in nonhematopoietic tissues. Isoform 4 is expressed in pancreatic alpha cells. -
Sequence similarities
Contains 2 C2 domains.
Contains 1 RabBD (Rab-binding) domain. -
Domain
The RabBD domain mediates interaction with RAB27A and recruitment on to vesicular structures in cytotoxic T-lymphocytes (CTL).
The C2 1 domain mediates binding to phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and localization to the cell membrane. -
Post-translational
modificationsIsoform 1 is highly susceptible to proteolytic degradation and is stabilized by the interaction with RAB27A. -
Cellular localization
Cell membrane. In the pancreatic alpha cells distributed in both peripheral and anterior regions. Localizes on the glucagon granules in the cell periphery and Cytoplasm. Cell membrane. Recruited on vesicular structures in cytotoxic T-lymphocytes (CTL) by RAB27A. - Information by UniProt
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Database links
- Entrez Gene: 54843 Human
- Entrez Gene: 83671 Mouse
- Omim: 612880 Human
- SwissProt: Q9HCH5 Human
- SwissProt: Q99N50 Mouse
- Unigene: 369520 Human
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Alternative names
- Breast cancer-associated antigen SGA-72M antibody
- CHR11SYT antibody
- EXO4 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human spleen tissue stained for SYTL2/SLP2 with ab231133 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-SYTL2/SLP2 antibody (ab231133) at 1 µg/ml + Mouse cerebrum tissue lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution -
Anti-SYTL2/SLP2 antibody (ab231133) at 2 µg/ml + Pig cerebrum tissue lysate
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Anti-SYTL2/SLP2 antibody (ab231133) at 2 µg/ml + Recombinant human SYTL2/SLP2
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231133 has been referenced in 1 publication.
- Zhou X et al. Long noncoding RNA BSN-AS2 induced by E2F1 promotes spinal osteosarcoma progression by targeting miR-654-3p/SYTL2 axis. Cancer Cell Int 20:133 (2020). PubMed: 32351327