Overview
Product nameSYVN1 protein (Active)See all SYVN1 proteins and peptides ...
Protein descriptionRecombinant fragment containing residues 246-617 of Human SYVN1; consisting of the complete C terminal cytoplasmic portion. The N terminal transmembrane spanning portion has been deleted. Contains a SUMO tag; 39.1kDa (without tag). (UniProt ID = Q86TM6).
Uniprot accessionQ86TM6
Molecular weight39.300kDa
Protein length372 amino acids
Expression hostE. coli
Properties
Biological activityThis N terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains. Typical enzyme concentration of 20-1000nM is used for in vitro conjugation depending on assay conditions.
E.C. Number6.3.2.-
FormLiquid
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferConstituents: 89% PBS, 10% Glycerol
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab125740
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| Functional Studies |
FuncS: Use at an assay dependent dilution. |
Protein info
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Alternative names
1200010C09RikDER3E3 ubiquitin protein ligase synoviolin
E3 ubiquitin-protein ligase synoviolinHMG coA reductase degradation 1 homologHRD1KIAA1810MGC40372OTTHUMP00000230429OTTHUMP00000230430OTTHUMP00000230431OTTHUMP00000230432Synovial apoptosis inhibitor 1Synovial apoptosis inhibitor 1, synoviolinSYNOVIOLINSynoviolin 1 isoform bSYVN1SYVN1_HUMAN
see all
FunctionActs as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
Tissue specificityUbiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the HRD1 family.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger is required for E3 ligase activity.
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated.
Cellular localizationEndoplasmic reticulum membrane.
References for SYVN1 protein (Active) (ab125740)
ab125740
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
