Anti-Sall4 antibody (ab29112)

Sehr geehrte Damen und Herren,
ich bin sehr an Ihrem Sall4 Antikoerper Anti-Sall4 antibody (ab29112) interessiert. Wir untersuchen verschiedene Sall4 Isoformen und muessten deshalb die Peptidsequenz des Epitops, welche Sie zur Generierung des Antikoerpers benutzt haben, wissen.
Es waere sehr nett und hilfreich, wenn Sie mir diese zukommen lassen koennten.
Ein herzliches Danke schoen im Voraus
Herzliche Gruesse

ANSWER:

Vielen Dank für Ihre Anfrage - in der Sprache, die Ihnen am liebsten ist: deutsch oder englisch; wir haben auch französische, spanische oder italienische Kollegen hier.

Die Immunogensequenz des Sall4-Antikörpers ab29112 lautet:

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Darf ich fragen, mit welcher Spezies Sie arbeiten? Wir garantieren, dass der Antikörper mit humanen und murinen Proben funktioniert. Aufgrund einer Immunogensequenzähnlichkeit von über 90% gegen Huhn und Xenopus gehen wir von einer Kreuzreaktivität aus, was allerdings noch nicht getestet wurde. Sollten Sie mit dem Ortholog von Primaten arbeiten, könnte der Antikörper ebenfalls kreuzreagieren, da eine 100%ige Übereinstimmung mit zum Beispiel Macaca mulatta oder Callithrix jacchus vorliegt. Sollten der Antikörper also für Ihre Versuchsvorhaben mit den verschiedenen Isoformen in Frage kommen und Sie mit einer der bislang ungetesteten Spezies arbeiten, können wir Ihnen ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie kostenlosen primären Antikörper, wenn Sie uns ein Abreview mit dem Testresultat zusenden.

Die genauen Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: http://www.abcam.com/collaborationdiscount.

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Would like to test these antibodies in ChIP.

ANSWER:

DISCOUNT CODES: *** Expiration date: April 16, 2012 These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in ChIP and include the corresponding code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Each code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Please note that only one code can be redeemed per order. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Hi,   Thanks. We have done experiments per your suggestions, but the results are not improved. Please see below:   GAPDH   SAII4   I am wondering if we can get the sales credit on this one.  

ANSWER:

I am sorry that the protocol tips did not prove to be successful. I have refunded the cost of ab29112. Your credit not number is ******. If there is anything else I can help you with, please let me know.

Dear Customer Service, I have successfully used the rabbit anti-Sall4 antibody for immunofluorescence and western blotting. I would be really interested in using this reagent for IP, and I would like to inquire if you have any experience using this antibody for this application?

ANSWER:

Thank you for your inquiry.

We are pleased to learn that you are happy with using the Sall4-antibody ab29112 in immunofluorescence (IF) and Western blot (WB). To our knowledge this antibody has not yet been tested in immunoprecipitation (IP). All tested applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. We do not see a reason why this antibody should not work in IP and would recommend testing it.

We also would like to encourage you to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints that can be used for discounts on future purchases. With Abreviews for each application (IF, WB and IP) you could earn up to 660 Abpoints which would be worth almost 150 USD on your next order. To find out more about our Abreview system, please see the following link: http://www.abcam.com/abreviews

I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

Hi, please provide us with tech support on ab29112. Details as below:   Cat.# ab29112   Anti-Sall4 antibody Order #: *-*****   Procedure Prepare 12%separating gel and 5% stacking gel. Dilute samples at least 1:4 with sample buffer, heat at 95 C for 4 minutes prior to loading. Applicate sample (200-300μg). Stop the Electrophoresis until bromphenol blue arrives at the bottom of the separating gel. In this process, 90V in the first 20 minutes, then 120V to the end. As the routine protocol for electrotransfer, 200mA for 2 hours. Block the NC membrane with 5% defatted milk powder in TBS/T for 1 hour, RT. Pour off blocking buffer. Add first antibody at 1:700 dilution in TBS/T to incubate the NC membrane overnight at 4 C. After washing the membrane using TBS/T in three times, incubate the second antibody at appropriate dilution in TBS/T at 37c for 1 hour. After washing the membrane using TBS/T for three times，we observed the straps in the gel imaging system.   We have repeated the experiments many times. The straps of GAPDH were very bright, while the straps for SALL4 could not be detected. In addition, the second antibody is OK in other experiments.  

ANSWER:

Thank you for contacting Abcam. I am sorry that you are having problems with ab29112. Based on the information you have provided and the information that is on our website for ab29112, I have two suggestions which may help: 1 - Use 3% BSA as a blocking and incubation agent, as we have evidence that his antibody will work well in a BSA block. 2 - Increase the primary antibody concentration from 1/700 to 1/500 and again incubate overnight at 4C. Please let me know if the above protocol tips do not prove to be successful and I will be happy to help further.