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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Sall4 antibody
See all Sall4 products (4) ...
Description
Rabbit polyclonal to Sall4
Tested applications
ChIP/Chip, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Mouse Sall4.
Positive control
E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab29112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP/Chip: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 85, 140 kDa (predicted molecular weight: 66, 113 kDa).
IHC-P: 1/2000
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Probable transcription factor.
Tissue specificity
Expressed in testis.
Involvement in disease
Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UJQ4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA407717 antibody
- AL022809 antibody
- AW536104 antibody
see all
Database links
- Entrez Gene: 57167 Human
- Entrez Gene: 99377 Mouse
- Entrez Gene: 398679 Xenopus laevis
- Omim: 607343 Human
- SwissProt: Q540H3 Human
- SwissProt: Q9UJQ4 Human
- SwissProt: A2AUZ8 Mouse
- SwissProt: Q6S7E8 Mouse
see all
Anti-Sall4 antibody images:
Immunocytochemistry/ Immunofluorescence - Sall4 antibody (ab29112)
ICC/IF image of ab29112 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29112, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Sall4 antibody (ab29112)
Anti-Sall4 antibody (ab29112) at 1 µg + E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 66, 113 kDa
Observed band size : 85,140 kDa (why is the actual band size different from the predicted?)
This antibody detects two bands which we believe correspond to the two isoforms of Sall4 outlined in Ma et al, 2006 (PMID: 16763212). This paper describes human isoforms running at 165 kDa and 95 kDa. In mouse embryonic stem cells we see both isoforms running at slightly lower molecular weights. We believe that these bands represent Sall4a (Swissprot: Q8BX22) and Sall4b (Swissprot: Q6S7E9) which are predicted to be 113 kDa and 66 kDa respectively.
Western blot - Sall4 antibody (ab29112)
Anti-Sall4 antibody (ab29112) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 66, 113 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 125 kDa (possible isoform).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sall4 antibody (ab29112)
IHC image of Sall4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29112, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Sall4 antibody (ab29112)
This product has been referenced in:
- Melton Cet al. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463:621-6 (2010). WB; Mouse.Read more (PubMed: 20054295) »
- Pardo Met al. An expanded Oct4 interaction network: implications for stem cell biology, development, and disease. Cell Stem Cell 6:382-95 (2010). WB; Mouse.Read more (PubMed: 20362542) »
See all 6 publications for this product
Publishing research using ab29112? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"