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Immunocytochemistry/ Immunofluorescence - Sall4 antibody (ab31968)
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Western blot - Sall4 antibody (ab31968)
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Western blot - Sall4 antibody (ab31968)
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Product Name
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Sall4 antibody
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See all Sall4 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Sall4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse Sall4.
(Peptide available as ab30772.)
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 113, 66 kDa).
Sall4 has been shown to migrate higher than would be predicted in SDS-PAGE in Ma et al 2006 (PMID: 16763212). This band would correspond to Sall4b.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome; also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in mice.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 99377 (Mouse)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.580 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Sall4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Sall4 antibody images, references, products related to ab31968 and other tools.
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Sall4 antibody images:
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 Immunocytochemistry/ Immunofluorescence - Sall4 antibody (ab31968)
ICC/IF image of ab31968 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31968, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Western blot - Sall4 antibody (ab31968)
Sall4 antibody (ab31968) at 1 µg/ml + E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 113, 66 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
This antibody detects a single band which we believe corresponds to Sall4b (Swissprot: Q6S7E9). The reason for the antibody failing to detect a band corresponding to Sall4a is unclear, although there is an extremely faint band at 140 kDa that may correspond to Sall4a. Ma et al, 2006 (PMID: 16763212) describes isoforms of Sall4 running significantly higher than would be predicted in SDS-PAGE (165 kDa and 95 kDa for Sall4a and Sall4b in human tissue, as opposed to predicted sizes of 113 kDa and 66 kDa). An alternative Sall4 antibody which detects both Sall4a and Sall4b is available as ab29112.
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Western blot - Sall4 antibody (ab31968)
All lanes : Sall4 antibody (ab31968) at 1/1000 dilution
Lane 1 : Mouse ES Cell Lysate
Lane 2 : Mouse ES Cell Lysate
Lane 3 : Mouse ES Cell Lysate
Lysates/proteins at 14 µg per lane.
Predicted band size : 113, 66 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa (possible isoform,non-specific binding),70 kDa (possible isoform,non-specific binding),85 kDa (possible isoform,non-specific binding).
The image shows detection of a Sall4 band at 145 kDa. Sall4 is known to run at a higher molecular weight than predicted by its primary amino acid sequence. The lower molecular weight bands may represent isoforms or non-specific binding. S. Rao, Harvard University
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Search PubMed (MEDLINE) for references to Sall4
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Sall4 antibody - more information
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