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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Sall4 antibody
See all Sall4 products (4) ...
Description
Rabbit polyclonal to Sall4
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse Sall4.
(Peptide available as ab30772.)
Positive control
E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 113, 66 kDa).
Sall4 has been shown to migrate higher than would be predicted in SDS-PAGE in Ma et al 2006 (PMID: 16763212). This band would correspond to Sall4b.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probable transcription factor.
Tissue specificity
Expressed in testis.
Involvement in disease
Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UJQ4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA407717 antibody
- AL022809 antibody
- AW536104 antibody
see all
Database links
- Entrez Gene: 99377 Mouse
- SwissProt: A2AUZ8 Mouse
- SwissProt: Q6S7E8 Mouse
- SwissProt: Q6S7E9 Mouse
- SwissProt: Q8BX22 Mouse
- SwissProt: Q99MV4 Mouse
- SwissProt: Q9CYI8 Mouse
- Unigene: 434054 Mouse
Anti-Sall4 antibody images:
Immunocytochemistry/ Immunofluorescence - Sall4 antibody (ab31968)
ICC/IF image of ab31968 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31968, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Sall4 antibody (ab31968)
Anti-Sall4 antibody (ab31968) at 1 µg/ml + E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 113, 66 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
This antibody detects a single band which we believe corresponds to Sall4b (Swissprot: Q6S7E9). The reason for the antibody failing to detect a band corresponding to Sall4a is unclear, although there is an extremely faint band at 140 kDa that may correspond to Sall4a. Ma et al, 2006 (PMID: 16763212) describes isoforms of Sall4 running significantly higher than would be predicted in SDS-PAGE (165 kDa and 95 kDa for Sall4a and Sall4b in human tissue, as opposed to predicted sizes of 113 kDa and 66 kDa). An alternative Sall4 antibody which detects both Sall4a and Sall4b is available as ab29112.
Western blot - Sall4 antibody (ab31968)
All lanes : Anti-Sall4 antibody (ab31968) at 1/1000 dilution
Lane 1 : Mouse ES Cell Lysate
Lane 2 : Mouse ES Cell Lysate
Lane 3 : Mouse ES Cell Lysate
Lysates/proteins at 14 µg per lane.
Predicted band size : 113, 66 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa (possible isoform,non-specific binding),70 kDa (possible isoform,non-specific binding),85 kDa (possible isoform,non-specific binding).
The image shows detection of a Sall4 band at 145 kDa. Sall4 is known to run at a higher molecular weight than predicted by its primary amino acid sequence. The lower molecular weight bands may represent isoforms or non-specific binding.
S. Rao, Harvard University
References for Anti-Sall4 antibody (ab31968)
ab31968 has not yet been referenced specifically in any publications.
Publishing research using ab31968? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"