Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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ab9465 has been referenced in 11 publications.
Publishing research using ab9465? Please let us know so that we can cite the reference in this datasheet
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ab9465 staining Sarcomeric Alpha Actinin in mouse heart tissue by Immunohistochemistry (Frozen sections). Serial longitudinal (12 µm) sections from wt hearts were incubated with ab9465. Nuclei were visualized with DAPI.
Image from Boyer JG et al, PLoS One. 2010 Mar 1;5(3):e9465, Fig 4.
All lanes : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/250 dilution
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 103 kDa
Observed band size : 103 kDa
Additional bands at : 25 kDa,57 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
ab9465 staining Sarcomeric Alpha Actinin in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Image from Liao IC et al, Biomaterials. 2011 Feb;32(6):1669-77. Epub 2010 Nov 16, Fig 3. DOI 10.1016/j.biomaterials.2010.10.049
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