Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Product Name 

Sarcomeric Alpha Actinin antibody [EA-53]

Product type 

Primary antibodies

Description 

Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin

Immunogen 

Purified rabbit skeletal alpha-actinin.

Reacts with 
(species key)

Ms, Rat, Cat, Chk, Cow, Dog, Fsh, Goat, Hm, Lzd, Pig, Rb, Shp, Snk, Xl

Specificity 

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin.
It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution from (PubMed:17383364).
IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
WB: 1 µg/ml.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Skeletal Muscle

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

Relevance 

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

EA-53

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

n/a

Form 

Liquid

Concentration 

0.125 mg/ml

Storage instructions 

Store at +4°C.

References for Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

  This product has been used in: (two most recent references)

Recher G et al. Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy. Opt Express 17:19763-77 (2009). IHC-Fr; Xenopus laevis. PubMed: 19997197

Zentilin L et al. Cardiomyocyte VEGFR-1 activation by VEGF-B induces compensatory hypertrophy and preserves cardiac function after myocardial infarction. FASEB J : (2009). PubMed: 20019242

See all 4 publication references for this product.


If you publish research using ab9465 please let us know so that we can cite the reference on this datasheet.

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