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Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

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Overview

Product name

Anti-Sarcomeric Alpha Actinin antibody [EA-53]
See all Sarcomeric Alpha Actinin products (8) ...

Description

Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin

Specificity

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

Tested applications

IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, IF, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Pig, Xenopus laevis, Fish, Lizard, Snake

Immunogen

Purified rabbit skeletal alpha-actinin.

Positive control

Skeletal Muscle

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

EA-53

Myeloma

unknown

Isotype

IgG1

Light chain type

unknown

  • Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image (enlarge)

  • Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Tissue specificity

Expressed in both skeletal and cardiac muscle.

Involvement in disease

Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Sequence similarities

Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.

Cellular localization

Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.

Target information above from: UniProt accessionP35609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Actin binding protein antibody
  • Actinin alpha 2 antibody
  • ACTN 2 antibody
  • ACTN2 antibody
  • ACTN2_HUMAN antibody
  • Alpha actinin 2 antibody
  • Alpha actinin skeletal muscle antibody
  • Alpha actinin skeletal muscle isoform 2 antibody
  • Alpha-actinin skeletal muscle isoform 2 antibody
  • Alpha-actinin-2 antibody
  • CMD1AA antibody
  • F actin cross linking protein antibody
  • F-actin cross-linking protein antibody
see all

Anti-Sarcomeric Alpha Actinin antibody [EA-53] images:

  Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

ab9465 staining Sarcomeric Alpha Actinin in mouse heart tissue by Immunohistochemistry (Frozen sections). Serial longitudinal (12 µm) sections from wt hearts were incubated with ab9465. Nuclei were visualized with DAPI.

Image from Boyer JG et al, PLoS One. 2010 Mar 1;5(3):e9465, Fig 4.

  Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/250 dilution

Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 103 kDa
Observed band size : 103 kDa
Additional bands at : 25 kDa,57 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 90 seconds

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

ab9465 staining Sarcomeric Alpha Actinin in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Image from Liao IC et al, Biomaterials. 2011 Feb;32(6):1669-77. Epub 2010 Nov 16, Fig 3. DOI 10.1016/j.biomaterials.2010.10.049

References for Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

This product has been referenced in:

  • Burridge PWet al. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One 6:e18293 (2011). ICC/IF; Human.Read more (PubMed: 21494607) »
  • Asumda FZ & Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol 12:44 (2011).Read more (PubMed: 21992089) »

See all 10 publications for this product

Publishing research using ab9465? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"