![Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](#Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-2.jpg)
![Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](#Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-4.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](#Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-5.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Sarcomeric Alpha Actinin antibody [EA-53]
See all Sarcomeric Alpha Actinin products (8) ...
Description
Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
Specificity
This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.
Tested applications
IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Pig, Xenopus laevis, Fish, Lizard, Snake
Immunogen
Purified rabbit skeletal alpha-actinin.
Positive control
Skeletal Muscle
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
EA-53
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cardiovascular >> Heart >> Cardiac arrhythmias
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
-
Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
![Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](/ps/datasheet/images/9/ab9465/Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-2.jpg)
(enlarge)
-
Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
![Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](/ps/datasheet/images/9/ab9465/Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-4.jpg)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) image](/ps/datasheet/images/9/ab9465/Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-5.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/25 - 1/50.(ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.)
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution. (PubMed: 17383364)
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20019242)
IF: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Target
Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificity
Expressed in both skeletal and cardiac muscle.
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Target information above from: UniProt accessionP35609
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Database links
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- SwissProt: Q3ZC55 Cow
- SwissProt: Q9JI91 Mouse
- Unigene: 37638 Mouse
- Unigene: 3970 Rat
Anti-Sarcomeric Alpha Actinin antibody [EA-53] images:
Immunohistochemistry (Frozen sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
ab9465 staining Sarcomeric Alpha Actinin in mouse heart tissue by Immunohistochemistry (Frozen sections). Serial longitudinal (12 µm) sections from wt hearts were incubated with ab9465. Nuclei were visualized with DAPI.
Image from Boyer JG et al, PLoS One. 2010 Mar 1;5(3):e9465, Fig 4.
Western blot - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
All lanes : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/250 dilution
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 103 kDa
Observed band size : 103 kDa
Additional bands at : 25 kDa,57 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
ab9465 staining Sarcomeric Alpha Actinin in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Image from Liao IC et al, Biomaterials. 2011 Feb;32(6):1669-77. Epub 2010 Nov 16, Fig 3. DOI 10.1016/j.biomaterials.2010.10.049
References for Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
This product has been referenced in:
- Burridge PWet al. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One 6:e18293 (2011). ICC/IF; Human.Read more (PubMed: 21494607) »
- Asumda FZ & Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol 12:44 (2011).Read more (PubMed: 21992089) »
See all 10 publications for this product
Publishing research using ab9465? Please let us know so that we can cite the reference in this datasheet
![Sarcomeric Alpha Actinin antibody [EA-53] for ICC/IF in Mouse (9465)](/ps/datasheet/images/9/ab9465/Sarcomeric-Alpha-Actinin-Primary-antibodies-ab9465-1.GIF)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"