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Sarcosine Assay Kit (ab65338)

Q&A (2)

Overview

  • Product nameSarcosine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (?max = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1-10000 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceSarcosine (N-methylglycine) is an intermediate and byproduct in the synthesis and degradation of glycine. Sarcosine is metabolized to glycine by sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a natural amino acid found in muscles and other body tissues and plays important role as an intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine.
    • Alternative names
        N methylglycine

    Applications

    Our Abpromise guarantee covers the use of ab65338 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
  • Application notesFuncS: Use at an assay dependent dilution.
    Note: Urine samples do not work well with the assay due to sample interferences.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Sarcosine Assay Kit images

    • Functional Studies - Sarcosine Assay Kit (ab65338)
      Functional Studies - Sarcosine Assay Kit (ab65338)
      Sarcosine Standard Curve performed using ab65338.

    Protocols

    References for Sarcosine Assay Kit (ab65338)

    ab65338 has not yet been referenced specifically in any publications.

    Publishing research using ab65338 ? Please let us know so that we can cite the reference in this datasheet

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    As I explained over the phone last Friday we would like to include your Sacosine Assay Kit (ab65338) into a monograph. Our experts group is currently elaborating a monograph on Sodium lauroyl sacrosinate. Part of the monograph is a test for free sodium...

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    Thank you for your enquiry. We have contacted our source to request details of the composition of the assay buffer, enzyme mix and probe and I am sorry they have regrettably informed me that this is commercially sensitive information and they are ...

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    I have a few basic questions for this assay. What biospecimens can be used for this assay (urine, tissue, blood, etc.). How long can the samples be stored and still give useful results. And is there any published data using this assay?　Thanks,

    Thank you for your enquiry. Please find below the answers to your questions. 1. Biospecimens like body fluids (plasma/ serum), tissues, and cell culture samples can be used. However, urine samples will give interference with the assay. For cell...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"