RabMAb

Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Overview

  • Product nameAnti-Sca1 / Ly6A/E antibody [EPR3355]
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

    Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Mouse Sca1/Ly6A/E.

  • Positive control
    • Mouse kidney cell lysate; Mouse kidney tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • RelevanceSca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
    • Cellular localizationAttached to the membrane by a GPI anchor.
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly6a antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • TAP antibody
      see all

    Anti-Sca1 / Ly6A/E antibody [EPR3355] images

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution + Mouse kidney cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
    • Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

    References for Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

    This product has been referenced in:
    • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P ; Mouse . Read more (PubMed: 24278321) »
    • Basile LA  et al. HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates. PLoS One 7:e30434 (2012). IHC-P ; Mouse . Read more (PubMed: 22383962) »

    See all 2 Publications for this product

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    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"