Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
Anti-Sca1 / Ly6A/E antibody [D7] (Allophycocyanin)
See all Sca1 / Ly6A/E products (14) ...
Description
Rat monoclonal [D7] to Sca1 / Ly6A/E (Allophycocyanin)
Conjugation
Allophycocyanin
Specificity
ab25323 is specific for Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family.
Tested applications
Flow Cyt, WB, IP, Cellular activation, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS; Stabilizing agent
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
D7
Isotype
IgG2a
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> HSC markers
Cardiovascular >> Heart >> Cardiogenesis >> Stem cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Lineage Markers >> Ectoderm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
WB
IP
Cellular activation
IHC-Fr
Application notes
Cellular activation: Use at an assay dependent dilution.
Flow Cyt: Use 0.2µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
Cellular localization
Attached to the membrane by a GPI anchor.
Alternative names
- Ly6a antibody
- Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
- T cell activating protein antibody
- TAP antibody
Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
References for Anti-Sca1 / Ly6A/E antibody [D7] (Allophycocyanin) (ab25323)
This product has been referenced in:
- Ivanov Vet al. Regulation of nuclear factor-kappa B and activator protein-1 activities after stimulation of T cells via glycosylphosphatidylinositol-anchored Ly-6A/E. J Immunol 153:2394-406 (1994).Read more (PubMed: 7915738) »
- Flood PMet al. Inhibition of Ly-6A antigen expression prevents T cell activation. J Exp Med 172:115-20 (1990).Read more (PubMed: 2141625) »
See all 6 publications for this product
Publishing research using ab25323? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"