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Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
Anti-Sca1 / Ly6A/E antibody [D7] (Biotin)
See all Sca1 / Ly6A/E products (14) ...
Description
Rat monoclonal [D7] to Sca1 / Ly6A/E (Biotin)
Conjugation
Biotin
Specificity
This antibody is specific to Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family; Mr 18 kDa.
Tested applications
IHC-P, Flow Cyt, IHC-Fr, Cellular activation, IP, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation: IL2 dependent mouse T cell line CTLL.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Studies with this antibody suggest that Sca1 may be involved in B and T lymphocyte responses, and it appears that the antigen may be required for T cell receptor mediated T cell activation.
Clonality
Monoclonal
Clone number
D7
Isotype
IgG2a
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> HSC markers
Cardiovascular >> Heart >> Cardiogenesis >> Stem cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Lineage Markers >> Ectoderm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration. (PubMed: 21563860)
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
CellAct: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
Target
Relevance
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
Cellular localization
Attached to the membrane by a GPI anchor.
Alternative names
- Ly6a antibody
- Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
- T cell activating protein antibody
- TAP antibody
Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
References for Anti-Sca1 / Ly6A/E antibody [D7] (Biotin) (ab25196)
This product has been referenced in:
- Agrawal Vet al. Recruitment of progenitor cells by an extracellular matrix cryptic peptide in a mouse model of digit amputation. Tissue Eng Part A 17:2435-43 (2011). IHC-P; Mouse.Read more (PubMed: 21563860) »
- Agrawal Vet al. Epimorphic regeneration approach to tissue replacement in adult mammals. Proc Natl Acad Sci U S A 107:3351-5 (2010). ICC/IF; Mouse.Read more (PubMed: 19966310) »
See all 2 publications for this product
Publishing research using ab25196? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"