![Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031) image](#ab25031_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031) image](#Sca1-Ly6AE-Primary-antibodies-ab25031-1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Overview
Product name
Anti-Sca1 / Ly6A/E antibody [D7] (FITC)
See all Sca1 / Ly6A/E products (14) ...
Description
Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
Conjugation
FITC
Specificity
This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
Tested applications
ICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular activation, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Immunogen
IL2 dependent T cell line CTL-L (Mouse).
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
D7
Isotype
IgG2a
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> HSC markers
Cardiovascular >> Heart >> Cardiogenesis >> Stem cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Lineage Markers >> Ectoderm
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
Flow Cyt: Use 1µg for 106 cells.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
IHC-Fr: Use at an assay dependent dilution.
CellAct: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration. (PubMed: 21858462)
Target
Relevance
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
Cellular localization
Attached to the membrane by a GPI anchor.
Alternative names
- Ly6A.2 antibody
- Ly6A/E antibody
- Ly6E.1 antibody
see all
Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
Anti-Sca1 / Ly6A/E antibody [D7] (FITC) images:
Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
![Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)](/ps/datasheet/Images/25/ab25031/ab25031_1.jpg)
BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
![Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)](/ps/datasheet/images/25/ab25031/Sca1-Ly6AE-Primary-antibodies-ab25031-1.jpg)
ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
This product has been referenced in:
- Mahdipour Eet al. Hoxa3 promotes the differentiation of hematopoietic progenitor cells into proangiogenic Gr-1+CD11b+ myeloid cells. Blood 117:815-26 (2011). Flow Cyt; Mouse.Read more (PubMed: 20974673) »
- Agrawal Vet al. Recruitment of progenitor cells by an extracellular matrix cryptic peptide in a mouse model of digit amputation. Tissue Eng Part A 17:2435-43 (2011). Flow Cyt; Mouse.Read more (PubMed: 21563860) »
See all 5 publications for this product
Publishing research using ab25031? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"