Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Product Name 

Sca1 / Ly6A/E antibody [D7] (FITC)

Product type 

Primary antibodies

Description 

Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)

Immunogen 

IL2 dependent T cell line CTL-L (Mouse).

Reacts with 
(species key)

Ms

Specificity 

This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.

Tested applications 
(see key)

CellAct, Flow Cyt, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
In vitro lymphocyte activation: Use at an assay dependent dilution.
Flow Cyt: Use <1µg for 10⁶ cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Attached to the membrane by a GPI anchor.

Research areas 

Developmental Biology >> Organogenesis >> Hematopoietic system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> HSC markers
Cardiovascular >> Heart >> Cardiogenesis >> Stem cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Lineage Markers >> Ectoderm
Immunology >> Adaptive Immunity >> T Cells >> Non-CD

Relevance 

Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

D7

Isotype 

IgG2a

Light chain type 

kappa

Storage buffer 

Preservative: Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C in the dark. Do not freeze.

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