|
|
|
|
Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
![Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)](/ps/datasheet/images/ab25031_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Sca1 / Ly6A/E antibody [D7] (FITC)
|
| |
See all Sca1 / Ly6A/E antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
|
|
Immunogen
|
IL2 dependent T cell line CTL-L (Mouse).
|
|
Reacts with
(species key)
|
Ms
|
|
Specificity
|
This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
|
|
Tested applications
(see key)
|
CellAct, Flow Cyt, IHC-Fr, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
In vitro lymphocyte activation: Use at an assay dependent dilution.
Flow Cyt: Use <1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Attached to the membrane by a GPI anchor.
|
|
Research areas
|
Developmental Biology >> Organogenesis >> Hematopoietic system development
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> HSC markers
Cardiovascular >> Heart >> Cardiogenesis >> Stem cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Lineage Markers >> Ectoderm
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
|
|
Relevance
|
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
|
|
|
Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rat
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
D7
|
|
Isotype
|
IgG2a
|
|
Light chain type
|
kappa
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Conjugation
|
Fluorescein
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C in the dark. Do not freeze.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Sca1 / Ly6A/E antibody [D7] (FITC) is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Sca1 / Ly6A/E antibody [D7] (FITC) images, references, products related to ab25031 and other tools.
|
| |
Sca1 / Ly6A/E antibody [D7] (FITC) images:
|
 Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Sca1 / Ly6A/E
|
| |
Sca1 / Ly6A/E antibody [D7] (FITC) - more information
|
Customer reviews (feedback) regarding Sca1 / Ly6A/E antibody [D7] (FITC) |
Customer FAQs regarding Sca1 / Ly6A/E antibody [D7] (FITC) |
Protocols for Sca1 / Ly6A/E antibody [D7] (FITC) |
Price and availability of products related to Sca1 / Ly6A/E antibody [D7] (FITC) |
| |
Sca1 / Ly6A/E antibody [D7] (FITC) - related products:
|
|
|
| |
Sca1 / Ly6A/E antibody [D7] (FITC) - other tools:
|
Contact Abcam with a Technical Enquiry about Sca1 / Ly6A/E antibody [D7] (FITC) |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)](/index.html?datasheet=25031#ab25031_1.jpg)
