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Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

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Overview

Product name

Anti-Sca1 / Ly6A/E antibody [D7] (FITC)
See all Sca1 / Ly6A/E products (14) ...

Description

Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)

Conjugation

FITC

Specificity

This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.

Tested applications

ICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular activation, IHC-Pmore details

Cross reactivity

Reacts with

Mouse

Immunogen

IL2 dependent T cell line CTL-L (Mouse).

Properties

Form

Liquid

Storage instructions

Store at +4°C in the dark. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

D7

Isotype

IgG2a

Light chain type

kappa

  • Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.

Cellular localization

Attached to the membrane by a GPI anchor.

Alternative names

  • Ly6A.2 antibody
  • Ly6A/E antibody
  • Ly6E.1 antibody
  • Lymphocyte antigen 6 complex locus A antibody
  • Lymphocyte antigen 6 complex locus E antibody
  • Retinoic acid induced gene E antibody
  • RIGE antibody
  • Sca1 antibody
  • Sca2 antibody
  • Stem cell antigen 1 antibody
  • Stem cell antigen 2 antibody
  • T cell activating protein antibody
  • TAP antibody
  • Thymic shared antigen 1 antibody
  • TSA1 antibody
see all

Anti-Sca1 / Ly6A/E antibody [D7] (FITC) images:

  Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.

  Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

This product has been referenced in:

  • Mahdipour Eet al. Hoxa3 promotes the differentiation of hematopoietic progenitor cells into proangiogenic Gr-1+CD11b+ myeloid cells. Blood 117:815-26 (2011). Flow Cyt; Mouse.Read more (PubMed: 20974673) »
  • Agrawal Vet al. Recruitment of progenitor cells by an extracellular matrix cryptic peptide in a mouse model of digit amputation. Tissue Eng Part A 17:2435-43 (2011). Flow Cyt; Mouse.Read more (PubMed: 21563860) »

See all 5 publications for this product

Publishing research using ab25031? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"