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Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Abreviews (1)Q&A (1)Specific References (6)

Overview

  • Product nameAnti-Sca1 / Ly6A/E antibody [D7] (FITC)See all Sca1 / Ly6A/E primary antibodies ...
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
  • ConjugationFITC
  • SpecificityThis antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
  • Tested applicationsICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular activation, IHC-P more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    IL2 dependent T cell line CTL-L (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab25031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
Flow Cyt Flow Cyt: Use 1µg for 106 cells.
IP IP: Use at an assay dependent dilution.
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
Cellular activation CellAct: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent concentration. PubMed: 21858462

Target

  • RelevanceSca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
  • Cellular localizationAttached to the membrane by a GPI anchor.
  • Database links
    • Alternative names
        Ly6A.2 antibodyLy6A/E antibodyLy6E.1 antibody
        Lymphocyte antigen 6 complex locus A antibodyLymphocyte antigen 6 complex locus E antibodyRetinoic acid induced gene E antibodyRIGE antibodySca1 antibodySca2 antibodyStem cell antigen 1 antibodyStem cell antigen 2 antibodyT cell activating protein antibodyTAP antibodyThymic shared antigen 1 antibodyTSA1 antibody
      see all

    Anti-Sca1 / Ly6A/E antibody [D7] (FITC) images

    • Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
      Flow Cytometry - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
      BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
    • Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
      Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)
      ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

    This product has been referenced in:
    • Wang H  et al. Over-expression of PDGFR-ß promotes PDGF-induced proliferation, migration, and angiogenesis of EPCs through PI3K/Akt signaling pathway. PLoS One 7:e30503 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22355314) »
    • Mahdipour E  et al. Hoxa3 promotes the differentiation of hematopoietic progenitor cells into proangiogenic Gr-1+CD11b+ myeloid cells. Blood 117:815-26 (2011). Flow Cyt ; Mouse . Read more (PubMed: 20974673) »

    See all 6 Publications for this product

    Publishing research using ab25031 ? Please let us know so that we can cite the reference in this datasheet

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sca1 / Ly6A/E antibody [D7] (FITC)

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    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Bone marrow)
    Specification Bone marrow
    Fixative Formaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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    Submitted Apr 09 2012

    Further to my email below, could you also tell me if the following antibody would bind the cell surface of NIH-3T3 cells?   http://www.abcam.com/Sca1-Ly6A-E-antibody-D7-FITC-ab25031.html#ab25031_1.jpg       Good afternoon,   I am looking for FITC and PE-c...

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    Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"