Useful resources:
- Blocking with immunizing peptide protocol (BL)

- Buffers and stock solutions

- Fluorophore table

- Troubleshooting tips
Western blotting (WB) protocols:
- Western blotting (WB) a beginner's guide
- Stripping for reprobing
- Western blotting of phospho-proteins protocol & tips
- Nuclear fractionation protocol
- Histone extraction protocol

- Histone western blotting protocol
- Mitochondrial purification protocol
- Soluble (S100) mitochondrial fractionation protocol
- Determining if the antibody binds only phosphorylated proteins
- Having problems?
Download our detailed troubleshooting tips for WB
Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:
- IHC-Fr staining protocol

- IHC-P staining protocol

- ICC (IF) protocol

- Double immunofluorescence - sequential protocol

- Double immunofluorescence - simultaneous protocol

- Determining if antibody binds only phosphorylated protein

- Fixation and permeabilization for IHC and ICC

- GMA embedding for immunohistochemistry
- Mouse on mouse staining procedure

- Perfusion fixation protocol

- Troubleshooting tips

Chromatin Immunoprecipitation (ChIP) protocols:
Dot blot protocols:
ELISA protocols:
- Direct ELISA protocol using primary antibody

- Direct ELISA protocol using fluorescent substrate

- Indirect ELISA protocol using primary and secondary antibodies
- Sandwich ELISA protocol using a pair of primary antibodies

ELISPOT protocols:
Flow cytometry / FACS protocols:
- Flow cytometry - a guide / introduction
- Direct flow cytometry (FACS) protocol
- Indirect flow cytometry (FACS) protocol
- Flow cytometry intracellular staining protocol
- Flow cytometry recommended controls

- Red blood cell lysis protocol

- Having problems?
Download our detailed flow cytometry troubleshooting tips!
![Immunocytochemistry/ Immunofluorescence - Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)](/ps/datasheet/Images/51/ab51317/ap487083-ab51317-090319.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)](/ps/datasheet/images/51/ab51317/Sca1-Ly6AE-Primary-antibodies-ab51317-10.jpg)
![Sca1 / Ly6A/E antibody [E13 161-7] for IHC-P in Mouse (51317)](/ps/datasheet/images/51/ab51317/Sca1-Ly6AE-Primary-antibodies-ab51317-6.jpg)
![Sca1 / Ly6A/E antibody [E13 161-7] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (51317)](/ps/datasheet/images/51/ab51317/Sca1-Ly6AE-Primary-antibodies-ab51317-9.jpg)
![Anti-Sca1 / Ly6A/E antibody [E13 161-7] for Immunocytochemistry/ Immunofluorescence in Mouse (51317)](/ps/datasheet/images/51/ab51317/Sca1-Ly6AE-Primary-antibodies-ab51317-17.jpg)