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ab51317 has been referenced in 6 publications.
Publishing research using ab51317? Please let us know so that we can cite the reference in this datasheet
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ICC/IF image of ab51317 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
ab51317 staining Sca1/Ly6A/E in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20ºC; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4ºC. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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